| Literature DB >> 7557305 |
Abstract
Single 10-base primers were used to generate randomly amplified polymorphic DNA (RAPD) markers in the shittake mushroom, Lentinula edodes. Seven primers produced polymorphisms in all 15 strains tested, producing 12-19 bands ranging from 0.34 to 2.52 kb. Thirteen of the 15 strains had unique DNA fingerprints, whereas L. edodes ATCC 28759 and ATCC 28760 exhibited identical RAPD profiles for all the primers. Molecular-genetic markers obtained with the RAPD assay can be used to differentiate strains of L. edodes and have potential applicatiosn in mushroom breeding and strain improvement programs.Entities:
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Year: 1995 PMID: 7557305 DOI: 10.1111/j.1574-6968.1995.tb07747.x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN: 0378-1097 Impact factor: 2.742