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Literature DB >> 7543968

Dielectrophoretic separation and enrichment of CD34+ cell subpopulation from bone marrow and peripheral blood stem cells.

M S Talary¹, K I Mills, T Hoy, A K Burnett, R Pethig.

Abstract

Dielectrophoresis has been used to enrich selected cell subpopulations in a mixed cell population by exploiting differential dielectric properties. Six-fold enrichment of stem cells expressing the CD34+ antigen has been achieved for bone marrow samples and peripheral blood, without the requirement for initial chemical treatment associated with immunoadsorption techniques.

Entities: Disease Gene

Mesh：

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Year: 1995 PMID： 7543968 DOI： 10.1007/bf02523050

Source DB: PubMed Journal: Med Biol Eng Comput ISSN： 0140-0118 Impact factor: 2.602

5 in total

1. Membrane changes accompanying the induced differentiation of Friend murine erythroleukemia cells studied by dielectrophoresis.

Authors: P R Gascoyne; R Pethig; J P Burt; F F Becker
Journal: Biochim Biophys Acta Date: 1993-06-18

2. Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry.

Authors: J G Bender; K L Unverzagt; D E Walker; W Lee; D E Van Epps; D H Smith; C C Stewart; L B To
Journal: Blood Date: 1991-06-15 Impact factor: 22.113

3. Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation.

Authors: X B Wang; Y Huang; P R Gascoyne; F F Becker; R Hölzel; R Pethig
Journal: Biochim Biophys Acta Date: 1994-08-03

4. Separation of viable and non-viable yeast using dielectrophoresis.

Authors: G H Markx; M S Talary; R Pethig
Journal: J Biotechnol Date: 1994-01-15 Impact factor: 3.307

5. Applications of a new optical technique for measuring the dielectrophoretic behaviour of micro-organisms.

Authors: J A Price; J P Burt; R Pethig
Journal: Biochim Biophys Acta Date: 1988-02-17

5 in total

27 in total

1. Separation of submicron bioparticles by dielectrophoresis.

Authors: H Morgan; M P Hughes; N G Green
Journal: Biophys J Date: 1999-07 Impact factor: 4.033

2. Holding forces of single-particle dielectrophoretic traps.

Authors: J Voldman; R A Braff; M Toner; M L Gray; M A Schmidt
Journal: Biophys J Date: 2001-01 Impact factor: 4.033

3. Dielectrophoretic separation of mouse melanoma clones.

Authors: Ahmet C Sabuncu; Jie A Liu; Stephen J Beebe; Ali Beskok
Journal: Biomicrofluidics Date: 2010-06-16 Impact factor: 2.800

4. Dielectrophoretic separation of colorectal cancer cells.

Authors: Fang Yang; Xiaoming Yang; Hong Jiang; Phillip Bulkhaults; Patricia Wood; William Hrushesky; Guiren Wang
Journal: Biomicrofluidics Date: 2010-01-12 Impact factor: 2.800

Review 5. Blood-on-a-chip.

Authors: Mehmet Toner; Daniel Irimia
Journal: Annu Rev Biomed Eng Date: 2005 Impact factor: 9.590

6. Role of peroxide in AC electrical field exposure effects on friend murine erythroleukemia cells during dielectrophoretic manipulations.

Authors: X Wang; J Yang; P R Gascoyne
Journal: Biochim Biophys Acta Date: 1999-01-04