Literature DB >> 7538839

Nucleic acid affinity chromatography: preparation and characterization of double-stranded RNA agarose.

J O Langland1, S M Pettiford, B L Jacobs.   

Abstract

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl). poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.

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Year:  1995        PMID: 7538839     DOI: 10.1006/prep.1995.1004

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN: 1046-5928            Impact factor:   1.650


  14 in total

1.  RNase-assisted RNA chromatography.

Authors:  Gracjan Michlewski; Javier F Cáceres
Journal:  RNA       Date:  2010-06-22       Impact factor: 4.942

2.  Regulation of vaccinia virus E3 protein by small ubiquitin-like modifier proteins.

Authors:  José González-Santamaría; Michela Campagna; María Angel García; Laura Marcos-Villar; Dolores González; Pedro Gallego; Fernando Lopitz-Otsoa; Susana Guerra; Manuel S Rodríguez; Mariano Esteban; Carmen Rivas
Journal:  J Virol       Date:  2011-09-28       Impact factor: 5.103

3.  Species specificity of protein kinase r antagonism by cytomegalovirus TRS1 genes.

Authors:  Stephanie J Child; Greg Brennan; Jacquelyn E Braggin; Adam P Geballe
Journal:  J Virol       Date:  2012-01-25       Impact factor: 5.103

4.  Double-stranded RNA is a trigger for apoptosis in vaccinia virus-infected cells.

Authors:  K V Kibler; T Shors; K B Perkins; C C Zeman; M P Banaszak; J Biesterfeldt; J O Langland; B L Jacobs
Journal:  J Virol       Date:  1997-03       Impact factor: 5.103

5.  Double-stranded RNA binding by a heterodimeric complex of murine cytomegalovirus m142 and m143 proteins.

Authors:  Stephanie J Child; Laura K Hanson; Crystal E Brown; Deanna M Janzen; Adam P Geballe
Journal:  J Virol       Date:  2006-10       Impact factor: 5.103

6.  Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing.

Authors:  Brian N Griffith; Callee M Walsh; Wioletta Szeszel-Fedorowicz; Aaron T Timperman; Lisa M Salati
Journal:  Biochim Biophys Acta       Date:  2006-10-06

7.  Antagonism of the protein kinase R pathway by the guinea pig cytomegalovirus US22-family gene gp145.

Authors:  Craig J Bierle; Mark R Schleiss; Adam P Geballe
Journal:  Virology       Date:  2012-08-20       Impact factor: 3.616

8.  Double-stranded RNA binding by the human cytomegalovirus PKR antagonist TRS1.

Authors:  Craig J Bierle; Kathryn M Semmens; Adam P Geballe
Journal:  Virology       Date:  2013-04-17       Impact factor: 3.616

9.  Differential contributions of tacaribe arenavirus nucleoprotein N-terminal and C-terminal residues to nucleocapsid functional activity.

Authors:  Alejandra D'Antuono; Maria Eugenia Loureiro; Sabrina Foscaldi; Cristina Marino-Buslje; Nora Lopez
Journal:  J Virol       Date:  2014-04-02       Impact factor: 5.103

10.  Identification of a plant-encoded analog of PKR, the mammalian double-stranded RNA-dependent protein kinase.

Authors:  J O Langland; S Jin; B L Jacobs; D A Roth
Journal:  Plant Physiol       Date:  1995-07       Impact factor: 8.340

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