Literature DB >> 7538479

A rapid permeabilization procedure for accurate quantitative determination of beta-galactosidase activity in yeast cells.

F Kippert1.   

Abstract

A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or vortexing. This procedure is equally applicable to fresh and frozen samples. It is compared to earlier reported methods and found to be superior by being more accurate and less time-consuming.

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Year:  1995        PMID： 7538479     DOI： 10.1111/j.1574-6968.1995.tb07523.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  48 in total

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5.  Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule.

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7.  Transformation by bovine papillomavirus type 1 E6 is independent of transcriptional activation by E6.

Authors:  R Ned; S Allen; S Vande Pol
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8.  "Labile" heme critically regulates mitochondrial biogenesis through the transcriptional co-activator Hap4p in Saccharomyces cerevisiae.

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Journal:  J Biol Chem       Date:  2020-02-18       Impact factor: 5.157

9.  A novel yeast system for in vivo selection of recognition sequences: defining an optimal c-Myb-responsive element.

Authors:  T Berge; S L Bergholtz; K B Andersson; O S Gabrielsen
Journal:  Nucleic Acids Res       Date:  2001-10-15       Impact factor: 16.971

10.  Mutations in the yeast Myb-like protein Bas1p resulting in discrimination between promoters in vivo but notin vitro.

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