Literature DB >> 7537871

Splicing of a circular yeast pre-mRNA in vitro.

C A Schindewolf1, H Domdey.   

Abstract

We have tested the fate of a circularized synthetic pre-mRNA transcript in a whole cell splicing extract of Saccharomyces cerevisiae. Our results demonstrate that this circular precursor RNA is able to induce spliceosome formation in vitro and that the products of the following splicing reaction are the lariat-shaped intron, and a mature circular mRNA. Thus, it would appear that free 5' and/or 3' ends are not obligatory for a splicing reaction to occur, although we find its efficiency to be strongly influenced by the presence or lack of free ends. To our knowledge, this is the first demonstration that a circular pre-mRNA molecule is recognized as a suitable substrate by an eukaryotic mRNA splicing apparatus.

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Year:  1995        PMID： 7537871      PMCID： PMC306821          DOI： 10.1093/nar/23.7.1133

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  39 in total

1.  Synthesis of circular RNA in bacteria and yeast using RNA cyclase ribozymes derived from a group I intron of phage T4.

Authors:  E Ford; M Ares
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

2.  Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.

Authors:  B Ruskin; A R Krainer; T Maniatis; M R Green
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

Review 3.  RNA splicing. U2 fulfils a commitment.

Authors:  P E Hodges; J D Beggs
Journal:  Curr Biol       Date:  1994-03-01       Impact factor: 10.834

4.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

5.  Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.

Authors:  P J Grabowski; R A Padgett; P A Sharp
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

6.  Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.

Authors:  C J Langford; F J Klinz; C Donath; D Gallwitz
Journal:  Cell       Date:  1984-03       Impact factor: 41.582

7.  Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome.

Authors:  H D Madhani; C Guthrie
Journal:  Genes Dev       Date:  1994-05-01       Impact factor: 11.361

8.  Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

Authors:  R Ng; J Abelson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

9.  Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts.

Authors:  C J Langford; D Gallwitz
Journal:  Cell       Date:  1983-06       Impact factor: 41.582

10.  The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA.

Authors:  S Teigelkamp; M McGarvey; M Plumpton; J D Beggs
Journal:  EMBO J       Date:  1994-02-15       Impact factor: 11.598
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  8 in total

1.  Inverse splicing of a discontinuous pre-mRNA intron generates a circular exon in a HeLa cell nuclear extract.

Authors:  S Braun; H Domdey; K Wiebauer
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

2.  Circular RNAs from transcripts of the rat cytochrome P450 2C24 gene: correlation with exon skipping.

Authors:  P G Zaphiropoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-25       Impact factor: 11.205

3.  Use of an engineered ribozyme to produce a circular human exon.

Authors:  S Mikheeva; M Hakim-Zargar; D Carlson; K Jarrell
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

4.  Pre-mRNA topology is important for 3'-end formation in Saccharomyces cerevisiae and mammals.

Authors:  G Stumpf; A Goppelt; H Domdey
Journal:  Mol Cell Biol       Date:  1996-05       Impact factor: 4.272

5.  During in vivo maturation of eukaryotic nuclear mRNA, splicing yields excised exon circles.

Authors:  B Bailleul
Journal:  Nucleic Acids Res       Date:  1996-03-15       Impact factor: 16.971

6.  Exon skipping and circular RNA formation in transcripts of the human cytochrome P-450 2C18 gene in epidermis and of the rat androgen binding protein gene in testis.

Authors:  P G Zaphiropoulos
Journal:  Mol Cell Biol       Date:  1997-06       Impact factor: 4.272

7.  In vitro generation of a circular exon from a linear pre-mRNA transcript.

Authors:  C Schindewolf; S Braun; H Domdey
Journal:  Nucleic Acids Res       Date:  1996-04-01       Impact factor: 16.971

8.  Circular RNA 0001073 Attenuates Malignant Biological Behaviours in Breast Cancer Cell and Is Delivered by Nanoparticles to Inhibit Mice Tumour Growth.

Authors:  Ziying Yi; Yunhai Li; Yushen Wu; Beilei Zeng; Hongzhong Li; Guosheng Ren; Xiaoyi Wang
Journal:  Onco Targets Ther       Date:  2020-06-29       Impact factor: 4.147
  8 in total

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