Literature DB >> 7532539

Efficient elution of purified proteins from polyvinylidene difluoride membranes (Immobilon) after transfer from SDS-PAGE and their use as immunogens.

B Szewczyk1, D F Summers.   

Abstract

Following separation of proteins by SDS-PAGE, they are electroblotted onto polyvinylidene difluoride membranes (Immobilon). Protein bands of interest are excised, and the proteins are eluted from the membrane with detergent-containing buffers at pH 9.5. The method routinely yields recovery of 70-90%, and this is independent of protein molecular weight.

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Year:  1994        PMID： 7532539     DOI： 10.1007/bf02824805

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  9 in total

1.  Preparative elution of proteins blotted to Immobilon membranes.

Authors:  B Szewczyk; D F Summers
Journal:  Anal Biochem       Date:  1988-01       Impact factor: 3.365

2.  Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.

Authors:  B Szewczyk; W G Laver; D F Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

3.  Electrophoretic elution of proteins from polyacrylamide gel slices.

Authors:  P Strålfors; P Belfrage
Journal:  Anal Biochem       Date:  1983-01       Impact factor: 3.365

4.  Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.

Authors:  D A Hager; R R Burgess
Journal:  Anal Biochem       Date:  1980-11-15       Impact factor: 3.365

5.  Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.

Authors:  M W Hunkapiller; E Lujan; F Ostrander; L E Hood
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

6.  Recovery of concentrated protein samples from sodium dodecyl sulfate-polyacrylamide gels.

Authors:  G P Tuszynski; C H Damsky; J P Fuhrer; L Warren
Journal:  Anal Biochem       Date:  1977-11       Impact factor: 3.365

7.  The recovery of nitrocellulose-bound protein.

Authors:  P J Anderson
Journal:  Anal Biochem       Date:  1985-07       Impact factor: 3.365

8.  Protein recovery from gel slices by steady-state stacking: an apparatus for the simultaneous extraction and concentration of ten samples.

Authors:  N Y Nguyen; J DiFonzo; A Chrambach
Journal:  Anal Biochem       Date:  1980-07-15       Impact factor: 3.365

9.  Preparative elution of proteins from nitrocellulose membranes after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  B S Parekh; H B Mehta; M D West; R C Montelaro
Journal:  Anal Biochem       Date:  1985-07       Impact factor: 3.365
  9 in total
  1 in total

1.  Rat C-reactive protein activates the autologous complement system.

Authors:  Niubel Diaz Padilla; Wim K Bleeker; Yvonne Lubbers; Gemma M M Rigter; Gerard J Van Mierlo; Mohamed R Daha; C Erik Hack
Journal:  Immunology       Date:  2003-08       Impact factor: 7.397
  1 in total

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