Literature DB >> 4037293

The recovery of nitrocellulose-bound protein.

P J Anderson.   

Abstract

Conditions for the electroelution of protein from polyacrylamide gels to nitrocellulose and its subsequent recovery have been examined. A procedure is described whereby soluble material suitable for further analysis can be obtained. Nitrocellulose-bound protein is dissolved in acetone. It can be precipitated from solution in the presence of a carrier such as Polybrene in a form that can subsequently be solubilized in good yield.

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Year:  1985        PMID： 4037293     DOI： 10.1016/0003-2697(85)90634-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Efficient elution of purified proteins from polyvinylidene difluoride membranes (Immobilon) after transfer from SDS-PAGE and their use as immunogens.

Authors:  B Szewczyk; D F Summers
Journal:  Mol Biotechnol       Date:  1994-10       Impact factor: 2.695

2.  Outer Membrane Protein Heterogeneity within Pseudomonas fluorescens and P. putida and Use of an OprF Antibody as a Probe for rRNA Homology Group I Pseudomonads.

Authors:  L Kragelund; K Leopold; O Nybroe
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792

Review 3.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
  3 in total

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