Literature DB >> 7531745

Delayed-type hypersensitivity to a recombinant mycobacterial antigen, MPB64, in guinea pigs sensitized to Mycobacterium tuberculosis or Mycobacterium bovis BCG.

S Haga1, R Yamaguchi, S Nagai, K Matsuo, A Yamazaki, R M Nakamura.   

Abstract

Recombinant MPB64 (rMPB64), a mycobacterial antigen, was obtained from an Escherichia coli clone transformed with a recombinant expression vector, pMAL64c. The rMPB64 was examined for the activity to elicit delayed-type hypersensitivity (DTH) in guinea pigs injected with liver Mycobacterium tuberculosis H37Rv or live M. bovis BCG Tokyo. It was found that rMPB64 has the same reactivity as native MPB64 (nMPB64) or MPT64 (nMPT64) and the potency to elicit DTH was 13.4 times higher than that of PPD. Because MPB64 is secreted only by living M. tuberculosis and some strains of BCG, it is possible to use this antigen for the diagnosis of tuberculosis.

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Year:  1995        PMID： 7531745     DOI： 10.1002/jlb.57.2.221

Source DB:  PubMed          Journal:  J Leukoc Biol        ISSN： 0741-5400            Impact factor:   4.962


  8 in total

1.  Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle.

Authors:  H M Vordermeier; A Whelan; P J Cockle; L Farrant; N Palmer; R G Hewinson
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

2.  Differential T-cell recognition of native and recombinant Mycobacterium tuberculosis GroES.

Authors:  I Rosenkrands; K Weldingh; P Ravn; L Brandt; P Højrup; P B Rasmussen; A R Coates; M Singh; P Mascagni; P Andersen
Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

3.  Immunogenicity of Mycobacterium tuberculosis RD1 region gene products in infected cattle.

Authors:  A S Mustafa; P J Cockle; F Shaban; R G Hewinson; H M Vordermeier
Journal:  Clin Exp Immunol       Date:  2002-10       Impact factor: 4.330

4.  Development of diagnostic reagents to differentiate between Mycobacterium bovis BCG vaccination and M. bovis infection in cattle.

Authors:  H M Vordermeier; P C Cockle; A Whelan; S Rhodes; N Palmer; D Bakker; R G Hewinson
Journal:  Clin Diagn Lab Immunol       Date:  1999-09

5.  Mapping of the delayed-type hypersensitivity-inducing epitope of secreted protein MPT64 from Mycobacterium tuberculosis.

Authors:  T Oettinger; A Holm; I M Mtoni; A B Andersen; K Hasløov
Journal:  Infect Immun       Date:  1995-12       Impact factor: 3.441

6.  Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.

Authors:  A O Whelan; J C Hope; C J Howard; D Clifford; R G Hewinson; H M Vordermeier
Journal:  Infect Immun       Date:  2003-11       Impact factor: 3.441

7.  Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens.

Authors:  B M Buddle; N A Parlane; D L Keen; F E Aldwell; J M Pollock; K Lightbody; P Andersen
Journal:  Clin Diagn Lab Immunol       Date:  1999-01

8.  Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.

Authors:  M J Elhay; T Oettinger; P Andersen
Journal:  Infect Immun       Date:  1998-07       Impact factor: 3.441
  8 in total

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