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Literature DB >> 7529491

Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme.

C Harteneck¹, P Klatt, K Schmidt, B Mayer.

Abstract

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.

Entities: Chemical Disease Species

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Year: 1994 PMID： 7529491 PMCID： PMC1137388 DOI： 10.1042/bj3040683

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

26 in total

1. Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.

Authors: D S Bredt; S H Snyder
Journal: Proc Natl Acad Sci U S A Date: 1990-01 Impact factor: 11.205

2. Purification of a Ca2+/calmodulin-dependent nitric oxide synthase from porcine cerebellum. Cofactor-role of tetrahydrobiopterin.

Authors: B Mayer; M John; E Böhme
Journal: FEBS Lett Date: 1990-12-17 Impact factor: 4.124

3. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.

Authors: D S Bredt; P M Hwang; C E Glatt; C Lowenstein; R R Reed; S H Snyder
Journal: Nature Date: 1991-06-27 Impact factor: 49.962

Review 4. Nitric oxide: physiology, pathophysiology, and pharmacology.

Authors: S Moncada; R M Palmer; E A Higgs
Journal: Pharmacol Rev Date: 1991-06 Impact factor: 25.468

5. Cationic lipid-mediated co-transfection of insect cells.

Authors: D R Groebe; A E Chung; C Ho
Journal: Nucleic Acids Res Date: 1990-07-11 Impact factor: 16.971

6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

7. Novel exogenous heme-dependent expression of mammalian cytochrome P450 using baculovirus.

Authors: A Asseffa; S J Smith; K Nagata; J Gillette; H V Gelboin; F J Gonzalez
Journal: Arch Biochem Biophys Date: 1989-11-01 Impact factor: 4.013

8. Brain nitric oxide synthase is a biopterin- and flavin-containing multi-functional oxido-reductase.

Authors: B Mayer; M John; B Heinzel; E R Werner; H Wachter; G Schultz; E Böhme
Journal: FEBS Lett Date: 1991-08-19 Impact factor: 4.124

Review 9. Glutamate, nitric oxide and cell-cell signalling in the nervous system.

Authors: J Garthwaite
Journal: Trends Neurosci Date: 1991-02 Impact factor: 13.837

10. Evidence for a bidomain structure of constitutive cerebellar nitric oxide synthase.

Authors: E A Sheta; K McMillan; B S Masters
Journal: J Biol Chem Date: 1994-05-27 Impact factor: 5.157

13 in total

1. Characterization of bovine endothelial nitric oxide synthase as a homodimer with down-regulated uncoupled NADPH oxidase activity: tetrahydrobiopterin binding kinetics and role of haem in dimerization.

Authors: B M List; B Klösch; C Völker; A C Gorren; W C Sessa; E R Werner; W R Kukovetz; K Schmidt; B Mayer
Journal: Biochem J Date: 1997-04-01 Impact factor: 3.857

2. Nitric oxide-induced autoinhibition of neuronal nitric oxide synthase in the presence of the autoxidation-resistant pteridine 5-methyltetrahydrobiopterin.

Authors: A C Gorren; A Schrammel; C Riethmüller; K Schmidt; D Koesling; E R Werner; B Mayer
Journal: Biochem J Date: 2000-04-15 Impact factor: 3.857

3. Haem insertion, dimerization and reactivation of haem-free rat neuronal nitric oxide synthase.

Authors: B Hemmens; A C Gorren; K Schmidt; E R Werner; B Mayer
Journal: Biochem J Date: 1998-06-01 Impact factor: 3.857

4. Delineation of the arginine- and tetrahydrobiopterin-binding sites of neuronal nitric oxide synthase.

Authors: A Boyhan; D Smith; I G Charles; M Saqi; P N Lowe
Journal: Biochem J Date: 1997-04-01 Impact factor: 3.857

5. Allosteric modulation of rat brain nitric oxide synthase by the pterin-site enzyme inhibitor 4-aminotetrahydrobiopterin.

Authors: S Pfeiffer; A C Gorren; E Pitters; K Schmidt; E R Werner; B Mayer
Journal: Biochem J Date: 1997-12-01 Impact factor: 3.857

6. Suramin and the suramin analogue NF307 discriminate among calmodulin-binding sites.

Authors: M Klinger; E Bofill-Cardona; B Mayer; C Nanoff; M Freissmuth; M Hohenegger
Journal: Biochem J Date: 2001-05-01 Impact factor: 3.857

7. Overexpression of neuronal nitric oxide synthase in insect cells reveals requirement of haem for tetrahydrobiopterin binding.

Authors: B M List; P Klatt; E R Werner; K Schmidt; B Mayer
Journal: Biochem J Date: 1996-04-01 Impact factor: 3.857

8. Inhibition of neuronal nitric oxide synthase by 6-nitrocatecholamines, putative reaction products of nitric oxide with catecholamines under oxidative stress conditions.

Authors: A Palumbo; G Astarita; M d'Ischia
Journal: Biochem J Date: 2001-05-15 Impact factor: 3.857

Review 9. In search of a function for tetrahydrobiopterin in the biosynthesis of nitric oxide.

Authors: B Mayer; E R Werner
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1995-05 Impact factor: 3.000

10. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement for bioactivation to the free acid, NG-nitro-L-arginine.

Authors: S Pfeiffer; E Leopold; K Schmidt; F Brunner; B Mayer
Journal: Br J Pharmacol Date: 1996-07 Impact factor: 8.739