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Literature DB >> 7525160

Staining of human telomeres with primed in situ labeling (PRINS).

A J Therkelsen¹, A Nielsen, J Koch, J Hindkjaer, S Kølvraa.

Abstract

As described, the PRINS method is a very rapid and reliable way of staining human telomeres. To obtain the maximum frequency of stained telomeres, the primer (CCCTAA)7 should be used, although the average frequency never quite reaches 100%. The frequency is strongly dependent on the age of the individual, being significantly higher in children and newborns than in adults. A difference between the (CCCTAA)7 primer and the complementary primer is demonstrated and a possible explanation is proposed, namely, that gaps in the C-rich strand cause chain elongation termination after the addition of only one dTTP molecule.

Entities: Chemical Species

Mesh：

Substances：
DNA Primers

Year: 1995 PMID： 7525160 DOI： 10.1159/000133903

Source DB: PubMed Journal: Cytogenet Cell Genet ISSN： 0301-0171

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Cited

15 in total

1. PRINS analysis of the telomeric sequence in seven lemurs.

Authors: Y Go; G Rakotoarisoa; Y Kawamoto; A Randrianjafy; N Koyama; H Hirai
Journal: Chromosome Res Date: 2000 Impact factor: 5.239

2. Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37 degrees C: characterization of an endodiploid clone.

Authors: M Gerbal; P Fournier; P Barry; M Mariller; F Odier; G Devauchelle; M Duonor-Cerutti
Journal: In Vitro Cell Dev Biol Anim Date: 2000-02 Impact factor: 2.416

3. Molecular cytogenetics investigation of the telomeres in a case of Philadelphia positive B-ALL with a single telomere expansion.

Authors: K Krejcí; J Stentoft; J Koch
Journal: Neoplasia Date: 1999-12 Impact factor: 5.715

Review 4. Strategies for signal amplification in nucleic acid detection.

Authors: S C Andras; J B Power; E C Cocking; M R Davey
Journal: Mol Biotechnol Date: 2001-09 Impact factor: 2.695

5. Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining.

Authors: Alan K Meeker; Wesley R Gage; Jessica L Hicks; Inpakala Simon; Jonathan R Coffman; Elizabeth A Platz; Gerrun E March; Angelo M De Marzo
Journal: Am J Pathol Date: 2002-04 Impact factor: 4.307

10. Genetic cooperation between the Werner syndrome protein and poly(ADP-ribose) polymerase-1 in preventing chromatid breaks, complex chromosomal rearrangements, and cancer in mice.

Authors: Michel Lebel; Josée Lavoie; Isabelle Gaudreault; Marc Bronsard; Régen Drouin
Journal: Am J Pathol Date: 2003-05 Impact factor: 4.307

Staining of human telomeres with primed in situ labeling (PRINS).

1. PRINS analysis of the telomeric sequence in seven lemurs.

2. Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37 degrees C: characterization of an endodiploid clone.

3. Molecular cytogenetics investigation of the telomeres in a case of Philadelphia positive B-ALL with a single telomere expansion.

Review 4. Strategies for signal amplification in nucleic acid detection.

5. Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining.

6. The labeling efficiency of human telomeres is increased by double-strand PRINS.

7. Primed in situ labeling technique for subtelomeric rearrangements in 70 children with idiopathic mental retardation.

8. Extreme variant of the short arm of chromosome 15.

9. Mouse telomere analysis using an optimized primed in situ (PRINS) labeling technique.

10. Genetic cooperation between the Werner syndrome protein and poly(ADP-ribose) polymerase-1 in preventing chromatid breaks, complex chromosomal rearrangements, and cancer in mice.