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Literature DB >> 7524029

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.

F Kirpekar¹, E Nordhoff, K Kristiansen, P Roepstorff, A Lezius, S Hahner, M Karas, F Hillenkamp.

Abstract

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

Entities: Disease

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Year: 1994 PMID： 7524029 PMCID： PMC308382 DOI： 10.1093/nar/22.19.3866

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

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2. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.

Authors: J R Sampson; O C Uhlenbeck
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3. Kinetics and mechanism of the acid-catalyzed hydrolysis of some purine nucleosides.

Authors: J A Zoltewicz; D F Clark; T W Sharpless; G Grahe
Journal: J Am Chem Soc Date: 1970-03-25 Impact factor: 15.419

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Authors: U Pieles; W Zürcher; M Schär; H E Moser
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5. Matrix-assisted laser desorption/ionization of restriction enzyme-digested DNA.

Authors: K Tang; S L Allman; C H Chen; L Y Cháng; M Schell
Journal: Rapid Commun Mass Spectrom Date: 1994-02 Impact factor: 2.419

6. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
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7. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.

Authors: K Zinn; D DiMaio; T Maniatis
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8. Ion stability of nucleic acids in infrared matrix-assisted laser desorption/ionization mass spectrometry.

Authors: E Nordhoff; R Cramer; M Karas; F Hillenkamp; F Kirpekar; K Kristiansen; P Roepstorff
Journal: Nucleic Acids Res Date: 1993-07-25 Impact factor: 16.971

9. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.

Authors: J F Milligan; D R Groebe; G W Witherell; O C Uhlenbeck
Journal: Nucleic Acids Res Date: 1987-11-11 Impact factor: 16.971

10. Autogenous control: ribosomal protein L10-L12 complex binds to the leader sequence of its mRNA.

Authors: M Johnsen; T Christensen; P P Dennis; N P Fiil
Journal: EMBO J Date: 1982 Impact factor: 11.598

25 in total

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5. Rapid and accurate characterisation of short tandem repeats by MALDI-TOF analysis of endonuclease cleaved RNA transcripts.

Authors: Doris Seichter; Stefan Krebs; Martin Förster
Journal: Nucleic Acids Res Date: 2004-01-20 Impact factor: 16.971