Literature DB >> 8341593

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for the mass and sequence analysis of natural and modified oligonucleotides.

U Pieles1, W Zürcher, M Schär, H E Moser.   

Abstract

We report the analysis and characterization of natural and modified oligonucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The present technology was highly improved for this class of compounds by using a new matrix, 2,4,6-trihydroxy acetophenone, together with di- and triammonium salts of organic or inorganic acids to suppress peak broadening due to multiple ion adducts. This methodology can be used in combination with time dependent degradation of oligonucleotides by exonucleases as powerful tool to determine sequence compositions.

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Year:  1993        PMID： 8341593      PMCID： PMC309754          DOI： 10.1093/nar/21.14.3191

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared.

Authors:  E Nordhoff; A Ingendoh; R Cramer; A Overberg; B Stahl; M Karas; F Hillenkamp; P F Crain
Journal:  Rapid Commun Mass Spectrom       Date:  1992-12       Impact factor: 2.419

2.  Mass spectrometry of DNA mixtures by laser ablation from frozen aqueous solution.

Authors:  D M Schieltz; C W Chou; C W Luo; R M Thomas; P Williams
Journal:  Rapid Commun Mass Spectrom       Date:  1992-10       Impact factor: 2.419

3.  Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers.

Authors:  F Hillenkamp; M Karas; R C Beavis; B T Chait
Journal:  Anal Chem       Date:  1991-12-15       Impact factor: 6.986

4.  Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.

Authors:  M Karas; F Hillenkamp
Journal:  Anal Chem       Date:  1988-10-15       Impact factor: 6.986

5.  Fractionation of synthetic deoxyribopolynucleotides on silica gel thin-layer plates.

Authors:  J J Michniewicz; C P Bahl; K Itakura; N Katagiri; S A Narang
Journal:  J Chromatogr       Date:  1973-10-10

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Matrix-assisted laser desorption mass spectrometry of oligodeoxythymidylic acids.

Authors:  T Huth-Fehre; J N Gosine; K J Wu; C H Becker
Journal:  Rapid Commun Mass Spectrom       Date:  1992-03       Impact factor: 2.419

8.  Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.

Authors:  K J Wu; A Steding; C H Becker
Journal:  Rapid Commun Mass Spectrom       Date:  1993-02       Impact factor: 2.419
  8 in total
  73 in total

1.  Identification of single stranded regions of DNA by enzymatic digestion with matrix-assisted laser desorption/ionization analysis.

Authors:  W P Bartolini; C M Bentzley; M V Johnston; B S Larsen
Journal:  J Am Soc Mass Spectrom       Date:  1999-06       Impact factor: 3.109

2.  Rapid determination of short DNA sequences by the use of MALDI-MS.

Authors:  E Nordhoff; C Luebbert; G Thiele; V Heiser; H Lehrach
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

3.  Determination of photomodified oligodeoxynucleotides by exonuclease digestion, matrix-assisted laser desorption/ionization and post-source decay mass spectrometry.

Authors:  L K Zhang; Y Ren; D Rempel; J S Taylor; M L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2001-10       Impact factor: 3.109

4.  Analysis of short tandem repeat polymorphisms by electrospray ion trap mass spectrometry.

Authors:  S Hahner; A Schneider; A Ingendoh; J Mosner
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

5.  Matrix-assisted laser desorption/ionization mass spectrometry methods for oligodeoxynucleotides: improvements in matrix, detection limits, quantification, and sequencing.

Authors:  L K Zhang; M L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2000-10       Impact factor: 3.109

6.  Fragmentation pathway studies of oligonucleotides in matrix-assisted laser desorption/ionization mass spectrometry by charge tagging and H/D exchange.

Authors:  Chau-Wen Chou; Patrick A Limbach; Richard B Cole
Journal:  J Am Soc Mass Spectrom       Date:  2002-12       Impact factor: 3.109

7.  Identification of the mass-silent post-transcriptionally modified nucleoside pseudouridine in RNA by matrix-assisted laser desorption/ionization mass spectrometry.

Authors:  K G Patteson; L P Rodicio; P A Limbach
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

Review 8.  MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.

Authors:  Christian Jurinke; Paul Oeth; Dirk van den Boom
Journal:  Mol Biotechnol       Date:  2004-02       Impact factor: 2.695

9.  Incorporation of 2'-amido-nucleosides in oligodeoxynucleotides and oligoribonucleotides as a model for 2'-linked conjugates.

Authors:  C Hendrix; B Devreese; J Rozenski; A van Aerschot; A De Bruyn; J Van Beeumen; P Herdewijn
Journal:  Nucleic Acids Res       Date:  1995-01-11       Impact factor: 16.971

10.  The detection of intact double-stranded DNA by MALDI.

Authors:  P Lecchi; L K Pannell
Journal:  J Am Soc Mass Spectrom       Date:  1995-10       Impact factor: 3.109
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