Literature DB >> 7515074

Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.

K Hashinaka1, S Hashida, K Hirota, A Saitoh, A Nakata, H Shinagawa, S Oka, K Shimada, E Ishikawa.   

Abstract

Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.

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Year:  1994        PMID: 7515074      PMCID: PMC263130          DOI: 10.1128/jcm.32.3.819-822.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  10 in total

1.  Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids.

Authors:  P Thongcharoen; C Wasi; S Louisirirotchanakul; J Parry; J Connell; P Mortimer
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

2.  Detection of antibody to HIV-1 in urine.

Authors:  S Desai; H Bates; F J Michalski
Journal:  Lancet       Date:  1991-01-19       Impact factor: 79.321

3.  Field evaluation of immunoglobulin G antibody capture tests for HIV-1 and HIV-2 antibodies in African serum, saliva and urine.

Authors:  A H Klokke; D Ocheng; S E Kalluvya; A G Nicoll; U Laukamm-Josten; J V Parry; P P Mortimer; J A Connell
Journal:  AIDS       Date:  1991-11       Impact factor: 4.177

4.  Preliminary report: accurate assays for anti-HIV in urine.

Authors:  J A Connell; J V Parry; P P Mortimer; R J Duncan; K A McLean; A M Johnson; M H Hambling; J Barbara; C P Farrington
Journal:  Lancet       Date:  1990-06-09       Impact factor: 79.321

5.  Use of urine for HIV-1 antibody screening.

Authors:  K J Reagan; C C Lile; G W Book; Y Devash; D L Winslow; A Bincsik
Journal:  Lancet       Date:  1990-02-10       Impact factor: 79.321

6.  Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.

Authors:  A Adachi; H E Gendelman; S Koenig; T Folks; R Willey; A Rabson; M A Martin
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

7.  Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme.

Authors:  A Saitoh; H Iwasaki; A Nakata; A Adachi; H Shinagawa
Journal:  Microbiol Immunol       Date:  1990       Impact factor: 1.955

8.  Antibodies to human immunodeficiency virus type 1 in the urine specimens of HIV-1-seropositive individuals.

Authors:  Y Z Cao; B Hosein; W Borkowsky; M Mirabile; L Baker; D Baldwin; B J Poiesz; A E Friedman-Kien
Journal:  AIDS Res Hum Retroviruses       Date:  1989-06       Impact factor: 2.205

9.  Detection of antibody IgG to HIV-1 in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens for diagnosis of HIV-1 infection.

Authors:  S Hashida; K Hirota; K Hashinaka; A Saitoh; A Nakata; H Shinagawa; S Oka; K Shimada; J Mimaya; S Matsushita
Journal:  J Clin Lab Anal       Date:  1993       Impact factor: 2.352

10.  Sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG in serum using a synthetic peptide, Cys-env gp46(188-224), as antigen.

Authors:  T Kohno; I Sakoda; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1992       Impact factor: 2.352

  10 in total
  13 in total

1.  Rapid formation of the immune complexes on solid phase in the immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody IgGs to HIV-1.

Authors:  S Ishikawa; S Hashida; K Hashinaka; A Adachi; S Oka; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

2.  Rapid and ultrasensitive enzyme immunoassay (thin aqueous layer immune complex transfer enzyme immunoassay) for HIV-1 p24 antigen.

Authors:  S Ishikawa; S Hashida; K Hashinaka; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

3.  Immune complex transfer enzyme immunoassay for antibody IgM to HIV-1 p17 antigen.

Authors:  S Hashida; S Ishikawa; I Nishikata; K Hashinaka; S Oka; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

4.  Optimal conditions of immune complex transfer enzyme immunoassays for antibody IgGs to HIV-1 using recombinant p17, p24, and reverse transcriptase as antigens.

Authors:  S Hashida; S Ishikawa; K Hashinaka; I Nishikata; S Oka; K Shimada; A Saito; A Takamizawa; H Shinagawa; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

5.  Ultrasensitive and rapid enzyme immunoassay (thin aqueous layer immune complex transfer enzyme immunoassay) for antibody IgG to HIV-1 p17 antigen.

Authors:  S Ishikawa; S Hashida; K Hashinaka; A Adachi; S Oka; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

6.  More sensitive immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1 with shorter incubation time for immunoreactions and larger volumes of serum samples.

Authors:  S Ishikawa; S Hashida; K Hashinaka; M Kojima; A Saito; A Takamizawa; H Shinagawa; S Oka; K Shimada; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

7.  Potential of the immune complex transfer enzyme immunoassay for antigens and antibodies to improve the sensitivity and its limitations.

Authors:  E Ishikawa; S Ishikawa; S Hashida; K Hashinaka
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

Review 8.  More reliable diagnosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/or serum with highly sensitive and specific enzyme immunoassay (immune complex transfer enzyme immunoassay): a review.

Authors:  S Hashida; K Hashinaka; S Ishikawa; E Ishikawa
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

9.  Preparations of recombinant HIV-1 p66 antigen to improve the specificity of immune complex transfer enzyme immunoassay of antibody IgG to HIV-1 reverse transcriptase.

Authors:  K Hashinaka; I Nishikata; S Hashida; A Adachi; S Oka; E Ishikawa
Journal:  J Clin Lab Anal       Date:  2000       Impact factor: 2.352

10.  Immune complex transfer enzyme immunoassay that is more sensitive and specific than western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens.

Authors:  S Hashida; K Hashinaka; I Nishikata; S Oka; K Shimada; A Saito; A Takamizawa; H Shinagawa; S Yano; H Kojima
Journal:  Clin Diagn Lab Immunol       Date:  1995-09
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