| Literature DB >> 1971662 |
J A Connell1, J V Parry, P P Mortimer, R J Duncan, K A McLean, A M Johnson, M H Hambling, J Barbara, C P Farrington.
Abstract
Untreated urine specimens from 358 patients (344 attending genito-urinary medicine clinics, 14 haemophiliacs) and 353 blood donors were tested blind by a simple IgG-capture particle-adherence test (GACPAT) and a rapid IgG-capture enzyme-linked immunosorbent assay (GACELISA) for antibody to human immunodeficiency virus (anti-HIV). All 158 urine specimens from seropositive subjects were anti-HIV positive by GACPAT and 157 of them (99.4%) were positive by GACELISA. Tests on 553 urine specimens from seronegative subjects gave two repeatable false-positive reactions by GACPAT (0.4%) and none by GACELISA. By means of a modified procedure anti-gp160 was detected by commercial western blot in the urine of 44 of 45 seropositive subjects examined. IgG-capture assays will detect anti-HIV in unconcentrated urine and so allow a diagnosis in circumstances when blood sampling is impracticable.Entities:
Mesh：
Substances：
Year: 1990 PMID： 1971662 DOI： 10.1016/0140-6736(90)91245-6
Source DB: PubMed Journal: Lancet ISSN： 0140-6736 Impact factor: 79.321