Literature DB >> 7514859

Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.

T W North1, G L Hansen, Y Zhu, J A Griffin, C K Shih.   

Abstract

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

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Year:  1994        PMID： 7514859      PMCID： PMC284465          DOI： 10.1128/AAC.38.2.388

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  16 in total

1.  Immunologic and proteolytic analysis of HIV-1 reverse transcriptase structure.

Authors:  A L Ferris; A Hizi; S D Showalter; S Pichuantes; L Babe; C S Craik; S H Hughes
Journal:  Virology       Date:  1990-04       Impact factor: 3.616

2.  Clinical staging of feline immunodeficiency virus infection.

Authors:  T Ishida; I Tomoda
Journal:  Nihon Juigaku Zasshi       Date:  1990-06

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Purification of outer membrane proteins of the gram-negative bacterium Neisseria gonorrhoeae.

Authors:  R C Judd
Journal:  Anal Biochem       Date:  1988-09       Impact factor: 3.365

5.  Feline immunodeficiency virus, a model for reverse transcriptase-targeted chemotherapy for acquired immune deficiency syndrome.

Authors:  T W North; G L North; N C Pedersen
Journal:  Antimicrob Agents Chemother       Date:  1989-06       Impact factor: 5.191

6.  Direct comparisons of inhibitor sensitivities of reverse transcriptases from feline and human immunodeficiency viruses.

Authors:  T W North; R C Cronn; K M Remington; R T Tandberg
Journal:  Antimicrob Agents Chemother       Date:  1990-08       Impact factor: 5.191

7.  Characterization of reverse transcriptase from feline immunodeficiency virus.

Authors:  T W North; R C Cronn; K M Remington; R T Tandberg; R C Judd
Journal:  J Biol Chem       Date:  1990-03-25       Impact factor: 5.157

8.  Inhibition of reverse transcriptase from feline immunodeficiency virus by analogs of 2'-deoxyadenosine-5'-triphosphate.

Authors:  R C Cronn; K M Remington; B D Preston; T W North
Journal:  Biochem Pharmacol       Date:  1992-10-06       Impact factor: 5.858

9.  Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats.

Authors:  J K Yamamoto; E Sparger; E W Ho; P R Andersen; T P O'Connor; C P Mandell; L Lowenstine; R Munn; N C Pedersen
Journal:  Am J Vet Res       Date:  1988-08       Impact factor: 1.156

10.  Immunologic abnormalities in pathogen-free cats experimentally infected with feline immunodeficiency virus.

Authors:  C D Ackley; J K Yamamoto; N Levy; N C Pedersen; M D Cooper
Journal:  J Virol       Date:  1990-11       Impact factor: 5.103
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  5 in total

1.  A novel Met-to-Thr mutation in the YMDD motif of reverse transcriptase from feline immunodeficiency virus confers resistance to oxathiolane nucleosides.

Authors:  R A Smith; K M Remington; R M Lloyd; R F Schinazi; T W North
Journal:  J Virol       Date:  1997-03       Impact factor: 5.103

2.  Mutants of feline immunodeficiency virus resistant to 2',3'-dideoxy-2',3'-didehydrothymidine.

Authors:  Y Q Zhu; K M Remington; T W North
Journal:  Antimicrob Agents Chemother       Date:  1996-09       Impact factor: 5.191

3.  A novel point mutation at position 156 of reverse transcriptase from feline immunodeficiency virus confers resistance to the combination of (-)-beta-2',3'-dideoxy-3'-thiacytidine and 3'-azido-3'-deoxythymidine.

Authors:  R A Smith; K M Remington; B D Preston; R F Schinazi; T W North
Journal:  J Virol       Date:  1998-03       Impact factor: 5.103

4.  Feline immunodeficiency virus reverse transcriptase: expression, functional characterization, and reconstitution of the 66- and 51-kilodalton subunits.

Authors:  M Amacker; M Hottiger; U Hübscher
Journal:  J Virol       Date:  1995-10       Impact factor: 5.103

5.  Selection and characterization of a mutant of feline immunodeficiency virus resistant to 2',3'-dideoxycytidine.

Authors:  H K Medlin; Y Q Zhu; K M Remington; T R Phillips; T W North
Journal:  Antimicrob Agents Chemother       Date:  1996-04       Impact factor: 5.191
  5 in total

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