Literature DB >> 7510942

Detection of alcohol-tolerant hiochi bacteria by PCR.

T Nakagawa1, M Shimada, H Mukai, K Asada, I Kato, K Fujino, T Sato.   

Abstract

We report a sensitive and rapid method for detection of hiochi bacteria by PCR. This method involves the electrophoresis of amplified DNA. Nucleotide sequences of the spacer region between 16S and 23S rRNA genes of 11 Lactobacillus strains were identified by analysis of PCR products. Five primers were designed by analysis of similarities among these sequences. A single cell of Lactobacillus casei subsp. casei could be detected when purified genomic DNA was used as the template. When various cell concentrations of L. casei subsp. casei were added to 50 ml of pasteurized sake and the cells were recovered, the detection limit was about one cell. No discrete band was observed in electrophoresis after PCR when human, Escherichia coli, mycoplasma, Acholeplasma, yeast, or mold DNA was used as the template.

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Year:  1994        PMID： 7510942      PMCID： PMC201360          DOI： 10.1128/aem.60.2.637-640.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  The nucleotide sequence of 16S rRNA gene from Streptomyces lividans TK21.

Authors:  Y Suzuki; T Yamada
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

2.  Nucleotide sequence of Thermus thermophilus HB8 gene coding 16S rRNA.

Authors:  N V Murzina; D P Vorozheykina; N I Matvienko
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

3.  Production of mevalonic acid by fermentation.

Authors:  G Tamura; K Ando; K Kodama; K Arima
Journal:  Appl Microbiol       Date:  1968-07

4.  Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG.

Authors:  Y Suzuki; A Nagata; Y Ono; T Yamada
Journal:  J Bacteriol       Date:  1988-06       Impact factor: 3.490

5.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Pseudomonas aeruginosa.

Authors:  H Y Toschka; P Höpfl; W Ludwig; K H Schleifer; N Ulbrich; V A Erdmann
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

6.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.

Authors:  J Brosius; T J Dull; D D Sleeter; H F Noller
Journal:  J Mol Biol       Date:  1981-05-15       Impact factor: 5.469

7.  Determination of nucleotide sequences in DNA.

Authors:  F Sanger
Journal:  Science       Date:  1981-12-11       Impact factor: 47.728

8.  Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB.

Authors:  C J Green; G C Stewart; M A Hollis; B S Vold; K F Bott
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  Natural relationship between bacteroides and flavobacteria.

Authors:  W G Weisburg; Y Oyaizu; H Oyaizu; C R Woese
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

10.  Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum.

Authors:  M Iwami; A Muto; F Yamao; S Osawa
Journal:  Mol Gen Genet       Date:  1984
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  6 in total

1.  Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoes.

Authors:  P W Tooley; B A Bunyard; M M Carras; E Hatziloukas
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

2.  Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons.

Authors:  G W Tannock; A Tilsala-Timisjarvi; S Rodtong; J Ng; K Munro; T Alatossava
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

3.  Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.

Authors:  A Tilsala-Timisjärvi; T Alatossava
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

4.  16S-23S and 23S-5S intergenic spacer regions of Streptococcus thermophilus and Streptococcus salivarius, primary and secondary structure.

Authors:  M Nour; A Naimi; G Beck; C Branlant
Journal:  Curr Microbiol       Date:  1995-11       Impact factor: 2.188

5.  Adjacent-possible ecological niche: growth of Lactobacillus species co-cultured with Escherichia coli in a synthetic minimal medium.

Authors:  Kouhei Mizuno; Mamiko Mizuno; Mio Yamauchi; Aya J Takemura; Veronica Medrano Romero; Kazuya Morikawa
Journal:  Sci Rep       Date:  2017-10-16       Impact factor: 4.379

6.  Design of Primers for Evaluation of Lactic Acid Bacteria Populations in Complex Biological Samples.

Authors:  Qiangchuan Hou; Xiaoye Bai; Weicheng Li; Xu Gao; Faming Zhang; Zhihong Sun; Heping Zhang
Journal:  Front Microbiol       Date:  2018-08-31       Impact factor: 5.640
  6 in total

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