Literature DB >> 7501464

Direct selection of cDNAs using whole chromosomes.

S Rouquier1, B J Trask, S Taviaux, G van den Engh, S Diriong, G G Lennon, D Giorgi.   

Abstract

We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) and used to capture ds cDNAs by an improved magnetic bead capture protocol. To demonstrate the capabilities of this method, the selected cDNAs were used as probes in FISH experiments. The selected cDNA populations specifically painted chromosomes 17 or 19 on metaphase spreads. These results demonstrate that it is possible to do chromosome painting using cDNA probes and that this method is a means to rapidly select expressed sequences encoded by any portion of the genome.

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Year:  1995        PMID： 7501464      PMCID： PMC307398          DOI： 10.1093/nar/23.21.4415

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  34 in total

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Authors:  J B Lawrence; R H Singer; J A McNeil
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Authors:  A J Brookes; D J Porteous
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4.  Isolation of human transcribed sequences from human-rodent somatic cell hybrids.

Authors:  P Liu; R Legerski; M J Siciliano
Journal:  Science       Date:  1989-11-10       Impact factor: 47.728

5.  Isolation of cDNA clones using yeast artificial chromosome probes.

Authors:  P Elvin; G Slynn; D Black; A Graham; R Butler; J Riley; R Anand; A F Markham
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

6.  Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization.

Authors:  B J Trask; G van den Engh; M Christensen; H F Massa; J W Gray; M Van Dilla
Journal:  Somat Cell Mol Genet       Date:  1991-03

7.  Direct cloning of human transcripts with HnRNA from hybrid cell lines.

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Journal:  Science       Date:  1990-08-10       Impact factor: 47.728

8.  Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes.

Authors:  H Telenius; A H Pelmear; A Tunnacliffe; N P Carter; A Behmel; M A Ferguson-Smith; M Nordenskjöld; R Pfragner; B A Ponder
Journal:  Genes Chromosomes Cancer       Date:  1992-04       Impact factor: 5.006

9.  Exon amplification: a strategy to isolate mammalian genes based on RNA splicing.

Authors:  A J Buckler; D D Chang; S L Graw; J D Brook; D A Haber; P A Sharp; D E Housman
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

10.  Mapping of human chromosome Xq28 by two-color fluorescence in situ hybridization of DNA sequences to interphase cell nuclei.

Authors:  B J Trask; H Massa; S Kenwrick; J Gitschier
Journal:  Am J Hum Genet       Date:  1991-01       Impact factor: 11.025
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  4 in total

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3.  Chromatin dynamics is correlated with replication timing.

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4.  MMASS: an optimized array-based method for assessing CpG island methylation.

Authors:  Ashraf E K Ibrahim; Natalie P Thorne; Katie Baird; Nuno L Barbosa-Morais; Simon Tavaré; V Peter Collins; Andrew H Wyllie; Mark J Arends; James D Brenton
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  4 in total

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