Literature DB >> 7501438

Restriction endonuclease BsoFI is sensitive to the 5'-methylation of deoxycytidines in its recognition sequence.

H Deissler1, B Genç, W Doerfler.   

Abstract

The isoschizomeric restriction endonucleases Fnu4HI and BsoFI cleave DNA at 5'-GCdecreasesNGC-3' sequences. Fnu4HI has been shown to be inhibited by 5'-CG-3'methylation in the sequences 5'-GmCGGC-3' or 5'-GCGGmCG-3'. We have now investigated the methylation sensitivity of BsoFI by testing its activity on plasmid DNA 5'-CG-3' methylated with the M.SssI DNA methyltransferase or on synthetic (CGG)n repetitive oligodeoxyribonucleotides which have been partly or completely C methylated. The data demonstrate that BsoFI cannot cleave at its recognition sequence when it is completely 5'-CG-3' methylated. These enzymes have proven to be useful in analyses of the methylation status in (CGG)n repeats of the human genome.

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Year:  1995        PMID： 7501438      PMCID： PMC307372          DOI： 10.1093/nar/23.21.4227

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  6 in total

1.  Instability of a 550-base pair DNA segment and abnormal methylation in fragile X syndrome.

Authors:  I Oberlé; F Rousseau; D Heitz; C Kretz; D Devys; A Hanauer; J Boué; M F Bertheas; J L Mandel
Journal:  Science       Date:  1991-05-24       Impact factor: 47.728

2.  Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

Authors:  M McClelland; M Nelson; E Raschke
Journal:  Nucleic Acids Res       Date:  1994-09       Impact factor: 16.971

3.  Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome.

Authors:  A J Verkerk; M Pieretti; J S Sutcliffe; Y H Fu; D P Kuhl; A Pizzuti; O Reiner; S Richards; M F Victoria; F P Zhang
Journal:  Cell       Date:  1991-05-31       Impact factor: 41.582

4.  Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI).

Authors:  P Renbaum; D Abrahamove; A Fainsod; G G Wilson; S Rottem; A Razin
Journal:  Nucleic Acids Res       Date:  1990-03-11       Impact factor: 16.971

5.  Variation of the CGG repeat at the fragile X site results in genetic instability: resolution of the Sherman paradox.

Authors:  Y H Fu; D P Kuhl; A Pizzuti; M Pieretti; J S Sutcliffe; S Richards; A J Verkerk; J J Holden; R G Fenwick; S T Warren
Journal:  Cell       Date:  1991-12-20       Impact factor: 41.582

6.  Methylation analysis of CGG sites in the CpG island of the human FMR1 gene.

Authors:  R S Hansen; S M Gartler; C R Scott; S H Chen; C D Laird
Journal:  Hum Mol Genet       Date:  1992-11       Impact factor: 6.150
  6 in total
  2 in total

1.  Restriction endonuclease isoschizomers ItaI, BsoFI and Fsp4HI are characterised by differences in their sensitivities to CpG methylation.

Authors:  B H Ramsahoye; A K Burnett; C Taylor
Journal:  Nucleic Acids Res       Date:  1997-08-15       Impact factor: 16.971

2.  Cloning and characterization of the lactococcal plasmid-encoded type II restriction/modification system, LlaDII.

Authors:  A Madsen; J Josephsen
Journal:  Appl Environ Microbiol       Date:  1998-07       Impact factor: 4.792
  2 in total

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