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Literature DB >> 7500948

Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease.

M A Lagarkova¹, O V Iarovaia, S V Razin.

Abstract

The specificity of long-range fragmentation of human nucleolar genes by Bal 31 nuclease was studied using fractionation of cleavage products by pulsed field gel electrophoresis, followed by Southern hybridization. It was found that limited treatment of permeabilised cells with this nuclease results in accumulation of DNA scissions in matrix attachment areas. Consequently, chromosomal DNA loops and their oligomers are released from the genome.

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Year: 1995 PMID： 7500948 DOI： 10.1007/bf00290373

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

17 in total

1. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites.

Authors: P N Cockerill; W T Garrard
Journal: Cell Date: 1986-01-31 Impact factor: 41.582

2. A nuclease-derived fragment of metaphase DNA and its relationship to the replicon.

Authors: R W Clark; P O Tseng; J M Lechuga
Journal: Exp Cell Res Date: 1987-04 Impact factor: 3.905

3. Interaction of DNA with nuclear scaffolds in vitro.

Authors: E Izaurralde; J Mirkovitch; U K Laemmli
Journal: J Mol Biol Date: 1988-03-05 Impact factor: 5.469

4. Different topoisomerase II antitumor drugs direct similar specific long-range fragmentation of an amplified c-MYC gene locus in living cells and in high-salt-extracted nuclei.

Authors: I I Gromova; B Thomsen; S V Razin
Journal: Proc Natl Acad Sci U S A Date: 1995-01-03 Impact factor: 11.205

5. Identification of a nuclear protein matrix.

Authors: R Berezney; D S Coffey
Journal: Biochem Biophys Res Commun Date: 1974-10-23 Impact factor: 3.575

6. The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.

Authors: S V Razin; V L Mantieva; G P Georgiev
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease.

1. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites.

2. A nuclease-derived fragment of metaphase DNA and its relationship to the replicon.

3. Interaction of DNA with nuclear scaffolds in vitro.

4. Different topoisomerase II antitumor drugs direct similar specific long-range fragmentation of an amplified c-MYC gene locus in living cells and in high-salt-extracted nuclei.

5. Identification of a nuclear protein matrix.

6. The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.

7. Isolation and characterization of rat liver nuclear matrices containing high molecular weight deoxyribonucleic acid.

8. Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation.

9. Periodicity of DNA folding in higher order chromatin structures.

10. Characterization of nuclear structures containing superhelical DNA.

1. Higher order chromatin structures in maize and Arabidopsis.