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Literature DB >> 1002771

Characterization of nuclear structures containing superhelical DNA.

Abstract

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.

Entities: Chemical

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Year: 1976 PMID： 1002771 DOI： 10.1242/jcs.22.2.303

Source DB: PubMed Journal: J Cell Sci ISSN： 0021-9533 Impact factor: 5.285

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Cited

74 in total

1. Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration.

Authors: Apolinar Maya-Mendoza; Rolando Hernández-Muñoz; Patricio Gariglio; Armando Aranda-Anzaldo
Journal: Nucleic Acids Res Date: 2003-11-01 Impact factor: 16.971

2. Evidence for cross-linking DNA by bis-intercalators with rigid and extended linkers is provided by knotting and catenation.

Authors: N K Annan; P R Cook; S T Mullins; G Lowe
Journal: Nucleic Acids Res Date: 1992-03-11 Impact factor: 16.971

3. Precise localization of the alpha-globin gene cluster within one of the 20- to 300-kilobase DNA fragments released by cleavage of chicken chromosomal DNA at topoisomerase II sites in vivo: evidence that the fragments are DNA loops or domains.

Authors: S V Razin; P Petrov; R Hancock
Journal: Proc Natl Acad Sci U S A Date: 1991-10-01 Impact factor: 11.205

Characterization of nuclear structures containing superhelical DNA.

1. Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration.

2. Evidence for cross-linking DNA by bis-intercalators with rigid and extended linkers is provided by knotting and catenation.

3. Precise localization of the alpha-globin gene cluster within one of the 20- to 300-kilobase DNA fragments released by cleavage of chicken chromosomal DNA at topoisomerase II sites in vivo: evidence that the fragments are DNA loops or domains.

Review 4. New applications of the Comet assay: Comet-FISH and transcription-coupled DNA repair.

5. Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis.

6. Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease.

7. Visualization of elementary DNA replication units in human nuclei corresponding in size to DNA loop domains.

8. Identifying Nuclear Matrix-Attached DNA Across the Genome.

9. Activation of ADP-ribosyltransferase in polyamine-depleted mammalian cells.

10. Effect of salt-treatment on manually isolated polytene chromosomes from Chironomus tentans.