Literature DB >> 7498141

A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels.

M Swain1, N W Ross.   

Abstract

A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.

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Year:  1995        PMID： 7498141     DOI： 10.1002/elps.11501601159

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  27 in total

1.  DNA-protein cross-linking in nuclei of immature and mature chicken erythrocytes.

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2.  Simultaneous isolation of total cellular lipids and RNA from cultured cells.

Authors:  David Akopian; Jheem D Medh
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3.  Intraplastidial trafficking of a phage-type RNA polymerase is mediated by a thylakoid RING-H2 protein.

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Journal:  Proc Natl Acad Sci U S A       Date:  2008-06-20       Impact factor: 11.205

4.  Purification of intracellular compartments involved in antigen processing: a new method based on magnetic sorting.

Authors:  L A Perrin-Cocon; P N Marche; C L Villiers
Journal:  Biochem J       Date:  1999-02-15       Impact factor: 3.857

5.  Mutation of aspartic acid residues in the fructosyltransferase of Streptococcus salivarius ATCC 25975.

Authors:  D D Song; N A Jacques
Journal:  Biochem J       Date:  1999-11-15       Impact factor: 3.857

6.  Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation.

Authors:  Yan-Ting Wu; Yan Wu; Jun-Yu Zhang; Ning-Ning Hou; Ai-Xia Liu; Jie-Xue Pan; Jie-Yang Lu; Jian-Zhong Sheng; He-Feng Huang
Journal:  J Assist Reprod Genet       Date:  2015-01-18       Impact factor: 3.412

7.  Intestinal MUC2 mucin supramolecular topology by packing and release resting on D3 domain assembly.

Authors:  Harriet E Nilsson; Daniel Ambort; Malin Bäckström; Elisabeth Thomsson; Philip J B Koeck; Gunnar C Hansson; Hans Hebert
Journal:  J Mol Biol       Date:  2014-05-08       Impact factor: 5.469

8.  Proteomic analysis on the alteration of protein expression in the early-stage placental villous tissue of electromagnetic fields associated with cell phone exposure.

Authors:  Qiong Luo; Ying Jiang; Min Jin; Jian Xu; He-Feng Huang
Journal:  Reprod Sci       Date:  2013-02-18       Impact factor: 3.060

9.  A synthetic resilin is largely unstructured.

Authors:  Kate M Nairn; Russell E Lyons; Roger J Mulder; Stephen T Mudie; David J Cookson; Emmanuelle Lesieur; Misook Kim; Deborah Lau; Fiona H Scholes; Christopher M Elvin
Journal:  Biophys J       Date:  2008-06-27       Impact factor: 4.033

10.  Unfolding dynamics of the mucin SEA domain probed by force spectroscopy suggest that it acts as a cell-protective device.

Authors:  Thaher Pelaseyed; Michael Zäch; Asa C Petersson; Frida Svensson; Denny G A Johansson; Gunnar C Hansson
Journal:  FEBS J       Date:  2013-02-21       Impact factor: 5.542
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