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Literature DB >> 7497121

The polymerase chain reaction and branching processes.

Abstract

We construct a mathematical model for the polymerase chain reaction and its mutations using the theory of branching processes. Under this model we study the number of mutations in a randomly chosen sequence after n PCR cycles. A method for estimating the mutation is proposed and the variance of this estimator is studied. We also study the distribution of the Hamming distance between two randomly chosen sequences and a method for estimating the mutation rate based on pairwise differences is proposed.

Mesh：

Substances：
DNA Primers
DNA

Year: 1995 PMID： 7497121 DOI： 10.1089/cmb.1995.2.63

Source DB: PubMed Journal: J Comput Biol ISSN： 1066-5277 Impact factor: 1.479

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Cited

13 in total

1. Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites.

Authors: Deepali Shinde; Yinglei Lai; Fengzhu Sun; Norman Arnheim
Journal: Nucleic Acids Res Date: 2003-02-01 Impact factor: 16.971

The polymerase chain reaction and branching processes.

1. Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites.

2. Establishment of new mutations in changing environments.

3. Thermodynamic prediction of protein neutrality.

4. Protein stability promotes evolvability.

5. The optimal burst of mutation to create a phenotype.

6. Efficiency of DNA replication in the polymerase chain reaction.

Review 7. Mutant library construction in directed molecular evolution: casting a wider net.

8. Quantifying the adaptive potential of an antibiotic resistance enzyme.

9. A graphical simulation model of the entire DNA process associated with the analysis of short tandem repeat loci.

10. Evolution favors protein mutational robustness in sufficiently large populations.