Literature DB >> 7487014

Development of a PCR for identification of Naegleria fowleri from the environment.

S Kilvington1, J Beeching.   

Abstract

A species-specific PCR for the identification of Naegleria fowleri was developed. In sensitivity studies, 10 trophozoites or cysts and 1 trophozoite or cyst could be detected after 35 and 45 cycles, respectively. In conjunction with a rapid DNA isolation method, this PCR was used to identify N. fowleri directly from primary cultures of environmental samples.

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Year:  1995        PMID： 7487014      PMCID： PMC167677          DOI： 10.1128/aem.61.10.3764-3767.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

Review 1.  Genome structure and evolution of Naegleria and its relatives.

Authors:  C G Clark
Journal:  J Protozool       Date:  1990 Jul-Aug

2.  Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods.

Authors:  A K Bej; M H Mahbubani; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1991-02       Impact factor: 4.792

3.  Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.

Authors:  A K Bej; M H Mahbubani; J L Dicesare; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

Review 4.  Primary amoebic meningo-encephalitis. An appraisal of present knowledge.

Authors:  R F Carter
Journal:  Trans R Soc Trop Med Hyg       Date:  1972       Impact factor: 2.184

5.  Characterization of Naegleria species by restriction endonuclease digestion of whole-cell DNA.

Authors:  J F De Jonckheere
Journal:  Mol Biochem Parasitol       Date:  1987-05       Impact factor: 1.759

6.  Amplification of repetitive DNA for the specific detection of Naegleria fowleri.

Authors:  G L McLaughlin; M H Vodkin; H W Huizinga
Journal:  J Clin Microbiol       Date:  1991-02       Impact factor: 5.948

7.  Detection of Naegleria fowleri cysts in environmental samples by using a DNA probe.

Authors:  O Sparagano
Journal:  FEMS Microbiol Lett       Date:  1993-09-15       Impact factor: 2.742

8.  Differentiation of Naegleria fowleri and other Naegleriae by polymerase chain reaction and hybridization methods.

Authors:  O Sparagano
Journal:  FEMS Microbiol Lett       Date:  1993-07-01       Impact factor: 2.742

9.  Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction.

Authors:  A van Belkum; J De Jonckheere; W G Quint
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

10.  Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes.

Authors:  M H Mahbubani; A K Bej; M H Perlin; F W Schaefer; W Jakubowski; R M Atlas
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948
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  11 in total

1.  The identification of free-living environmental isolates of amoebae from Bulgaria.

Authors:  Nina Tsvetkova; Mark Schild; Stefan Panaiotov; Rossitza Kurdova-Mintcheva; Bruno Gottstein; Julia Walochnik; Horst Aspöck; Mar Siles Lucas; Norbert Müller
Journal:  Parasitol Res       Date:  2004-02-04       Impact factor: 2.289

2.  PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.

Authors:  Marc Schild; Christian Gianinazzi; Bruno Gottstein; Norbert Müller
Journal:  J Clin Microbiol       Date:  2006-11-22       Impact factor: 5.948

3.  Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis.

Authors:  Jonas Behets; Priscilla Declerck; Yasmine Delaedt; Lieve Verelst; Frans Ollevier
Journal:  Curr Microbiol       Date:  2006-11-13       Impact factor: 2.188

4.  Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment.

Authors:  Michel Pélandakis; Pierre Pernin
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

5.  Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri.

Authors:  Yvonne Qvarnstrom; Govinda S Visvesvara; Rama Sriram; Alexandre J da Silva
Journal:  J Clin Microbiol       Date:  2006-10       Impact factor: 5.948

6.  Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.

Authors:  Bret S Robinson; Paul T Monis; Phillip J Dobson
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

7.  Quantitative detection of the free-living amoeba Hartmannella vermiformis in surface water by using real-time PCR.

Authors:  Melanie W Kuiper; Rinske M Valster; Bart A Wullings; Harry Boonstra; Hauke Smidt; Dick van der Kooij
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

Review 8.  Waterborne protozoan pathogens.

Authors:  M M Marshall; D Naumovitz; Y Ortega; C R Sterling
Journal:  Clin Microbiol Rev       Date:  1997-01       Impact factor: 26.132

9.  The identification of Naegleria fowleri from water and soil samples by nested PCR.

Authors:  Rebecca C Maclean; Dennis J Richardson; Robin LePardo; Francine Marciano-Cabral
Journal:  Parasitol Res       Date:  2004-06       Impact factor: 2.289

10.  Detection of Balamuthia mandrillaris DNA by real-time PCR targeting the RNase P gene.

Authors:  Albrecht F Kiderlen; Elke Radam; Astrid Lewin
Journal:  BMC Microbiol       Date:  2008-12-03       Impact factor: 3.605
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