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Literature DB >> 17121998

PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.

Marc Schild¹, Christian Gianinazzi, Bruno Gottstein, Norbert Müller.

Abstract

We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 2006 PMID： 17121998 PMCID： PMC1828997 DOI： 10.1128/JCM.01357-06

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

28 in total

Review 1. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies.

Authors: U Lehmann; H Kreipe
Journal: Methods Date: 2001-12 Impact factor: 3.608

2. December 2002: 19-year old male with febrile illness after jet ski accident.

Authors: Eva Gyori
Journal: Brain Pathol Date: 2003-04 Impact factor: 6.508

3. Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment.

Authors: Michel Pélandakis; Pierre Pernin
Journal: Appl Environ Microbiol Date: 2002-04 Impact factor: 4.792

4. Organotypic slice cultures from rat brain tissue: a new approach for Naegleria fowleri CNS infection in vitro.

Authors: C Gianinazzi; M Schild; N Müller; S L Leib; F Simon; S Nuñez; P Joss; B Gottstein
Journal: Parasitology Date: 2005-12 Impact factor: 3.234

5. Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri.

Authors: Fabienne L Réveiller; Pierre-André Cabanes; Francine Marciano-Cabral
Journal: Parasitol Res Date: 2002-05 Impact factor: 2.289

6. Primary meningoencephalitis by Naegleria fowleri: first reported case from Mangalore, South India.

Authors: Shalini Shenoy; Godwin Wilson; H V Prashanth; K Vidyalakshmi; B Dhanashree; R Bharath
Journal: J Clin Microbiol Date: 2002-01 Impact factor: 5.948

7. Nitric oxide is protective in listeric meningoencephalitis of rats.

Authors: K A Remer; T W Jungi; R Fatzer; M G Täuber; S L Leib
Journal: Infect Immun Date: 2001-06 Impact factor: 3.441

8. Naegleria meningitis: a rare survival.

Authors: R Jain; S Prabhakar; M Modi; R Bhatia; R Sehgal
Journal: Neurol India Date: 2002-12 Impact factor: 2.117

9. PCR-based detection of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction.

Authors: N Müller; V Zimmermann; U Forster; M Bienz; B Gottstein; M Welle
Journal: Vet Parasitol Date: 2003-06-11 Impact factor: 2.738

10. Detection of Naegleria spp. and Naegleria fowleri: a comparison of flagellation tests, ELISA and PCR.

Authors: J Behets; F Seghi; P Declerck; L Verelst; L Duvivier; A Van Damme; F Ollevier
Journal: Water Sci Technol Date: 2003 Impact factor: 1.915

17 in total

Review 1. Cardiac involvement with parasitic infections.

Authors: Alicia Hidron; Nicholas Vogenthaler; José I Santos-Preciado; Alfonso J Rodriguez-Morales; Carlos Franco-Paredes; Anis Rassi
Journal: Clin Microbiol Rev Date: 2010-04 Impact factor: 26.132

2. Demonstration of Balamuthia and Acanthamoeba mitochondrial DNA in sectioned archival brain and other tissues by the polymerase chain reaction.

Authors: Shigeo Yagi; Frederick L Schuster; Govinda S Visvesvara
Journal: Parasitol Res Date: 2007-11-25 Impact factor: 2.289

3. Demonstration of Balamuthia and Acanthamoeba mitochondrial DNA in sectioned archival brain and other tissues by the polymerase chain reaction.

Authors: Shigeo Yagi; Frederick L Schuster; Govinda S Visvesvara
Journal: Parasitol Res Date: 2007-09-25 Impact factor: 2.289

4. Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay.

Authors: Po-Min Kao; Min-Che Tung; Bing-Mu Hsu; Ming-Yuan Chou; Hsiu-Wu Yang; Cheng-Yu She; Shu-Min Shen
Journal: Parasitol Res Date: 2013-02-21 Impact factor: 2.289