Literature DB >> 7430309

Isocratic high-performance liquid chromatography separation of phosphoglycerides and lysophosphoglycerides.

R W Gross, B E Sobel.   

Abstract

An isocratic, rapid method for separation of phospho- and lysophospholipids by high-performance liquid chromatography employing acetonitrile, methanol and water as the mobile phase is described. Separation is achieved on a silica based cation-exchange column with individual components monitored directly by UV absorbance at 203 nm. In solutions of phospho- and lysophospholipid standards and in extracts of rabbit myocardium, recovery of individual components exceeds 95%. With the exception of phosphatidyl serine and phosphatidyl ethanolamine that could not be resolved other major phospho- and lysophospholipid constituents found in extracts of cell membranes are separated completely within 40 min. Retention time for selected moieties can be shortened even further with flow programming.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7430309     DOI： 10.1016/s0021-9673(00)80538-5

Source DB:  PubMed          Journal:  J Chromatogr


  9 in total

1.  Factors influencing the electrospray intrasource separation and selective ionization of glycerophospholipids.

Authors:  Xianlin Han; Kui Yang; Jingyue Yang; Kora N Fikes; Hua Cheng; Richard W Gross
Journal:  J Am Soc Mass Spectrom       Date:  2006-01-18       Impact factor: 3.109

2.  The effect of inhibitors of platelet aggregation on the metabolism of platelet-activating factor (PAF) in washed rabbit platelets.

Authors:  C O'Neill; A J Ammit; R Korth; S Fleming; X Wells
Journal:  Lipids       Date:  1991-12       Impact factor: 1.880

3.  Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization.

Authors:  Xianlin Han; Kui Yang; Hua Cheng; Kora N Fikes; Richard W Gross
Journal:  J Lipid Res       Date:  2005-04-16       Impact factor: 5.922

4.  Increase by lysophosphatidylcholines of smooth muscle Ca2+ sensitivity in alpha-toxin-permeabilized small mesenteric artery from the rat.

Authors:  P E Jensen; J Ohanian; B Stausbøl-Grøn; N H Buus; C Aalkjaer
Journal:  Br J Pharmacol       Date:  1996-03       Impact factor: 8.739

5.  Electrophysiologic effects of intracellular lysophosphoglycerides and their accumulation in cardiac lymph with myocardial ischemia in dogs.

Authors:  H Akita; M H Creer; K A Yamada; B E Sobel; P B Corr
Journal:  J Clin Invest       Date:  1986-07       Impact factor: 14.808

Review 6.  Applications of mass spectrometry for cellular lipid analysis.

Authors:  Chunyan Wang; Miao Wang; Xianlin Han
Journal:  Mol Biosyst       Date:  2015-01-19

Review 7.  The evolution of lipidomics through space and time.

Authors:  Richard W Gross
Journal:  Biochim Biophys Acta Mol Cell Biol Lipids       Date:  2017-04-28       Impact factor: 4.698

8.  Prophylaxis of early ventricular fibrillation by inhibition of acylcarnitine accumulation.

Authors:  P B Corr; M H Creer; K A Yamada; J E Saffitz; B E Sobel
Journal:  J Clin Invest       Date:  1989-03       Impact factor: 14.808

9.  Shotgun lipidomics identifies a paired rule for the presence of isomeric ether phospholipid molecular species.

Authors:  Kui Yang; Zhongdan Zhao; Richard W Gross; Xianlin Han
Journal:  PLoS One       Date:  2007-12-26       Impact factor: 3.240
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.