Deacetylation of PS-5, a new beta-lactam compound. II. Separation and purification of L- and D-amino acid acylases from Pseudomonas sp. 1158.

L-Amino acid acylase and D-amino acid acylase of Pseudomonas sp. 1158 which converted PS-5 to NS-5 (deacetylated PS-5) were separated and purified by sonication, streptomycin and ammonium sulfate fractionations, DEAE-Sephacel column chromatography and gel filtration. Molecular weight and the isoelectric point were estimated to be 75,000 and pI 5.45 for L-amino acid acylase and 100,000 and pI 4.95 for D-amino acid acylase.