Literature DB >> 16348465

Purification and Characterization of d-Aminoacylase from Alcaligenes faecalis DA1.

Y B Yang1, C S Lin, C P Tseng, Y J Wang, Y C Tsai.   

Abstract

A d-aminoacylase from Alcaligenes faecalis DA1 has been purified to homogeneity by a simple purification procedure with two columns, Fractogel DEAE-650 and HW-50. The specific activity of the purified enzyme was found to be 580 U/mg of protein with N-acetyl-dl-methionine as the reaction substrate. The apparent molecular weight and isoelectric point of this enzyme were determined to be 55,000 and 5.4, respectively.

Entities:  

Year:  1991        PMID： 16348465      PMCID： PMC182879          DOI： 10.1128/aem.57.4.1259-1260.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  A modified ninhydrin colorimetric analysis for amino acids.

Authors:  H ROSEN
Journal:  Arch Biochem Biophys       Date:  1957-03       Impact factor: 4.013

2.  Occurrence of D-acylase in soil bacteria.

Authors:  Y KAMEDA; E TOYOURA; Y KIMURA
Journal:  Nature       Date:  1958-04-26       Impact factor: 49.962

3.  Production of d-Aminoacylase from Alcaligenes denitrificans subsp. xylosoxydans MI-4.

Authors:  M Moriguchi; K Ideta
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Production and Purification of d-Aminoacylase from Alcaligenes denitrificans and Taxonomic Study of the Strain.

Authors:  Y C Tsai; C P Tseng; K M Hsiao; L Y Chen
Journal:  Appl Environ Microbiol       Date:  1988-04       Impact factor: 4.792

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Deacetylation of PS-5, a new beta-lactam compound. II. Separation and purification of L- and D-amino acid acylases from Pseudomonas sp. 1158.

Authors:  K Kubo; T Ishikura; Y Fukagawa
Journal:  J Antibiot (Tokyo)       Date:  1980-06       Impact factor: 2.649

8.  Deacetylation of PS-5, a new beta-lactam compound. III. Enzymological characterization of L-amino acid acylase and D-amino acid acylase from Pseudomonas sp. 1158.

Authors:  K Kubo; T Ishikura; Y Fukagawa
Journal:  J Antibiot (Tokyo)       Date:  1980-06       Impact factor: 2.649

9.  Deacetylation of PS-5, a new beta-lactam compound, I. Microbial deacetylation of PS-5.

Authors:  Y Fukagawa; K Kubo; T Ishikura; K Kouno
Journal:  J Antibiot (Tokyo)       Date:  1980-06       Impact factor: 2.649
  9 in total
  2 in total

1.  Structural-based mutational analysis of D-aminoacylase from Alcaligenes faecalis DA1.

Authors:  Cheng-Sheng Hsu; Wen-Lin Lai; Wei-Wei Chang; Shwu-Huey Liaw; Ying-Chieh Tsai
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

Review 2.  Application and microbial preparation of D-valine.

Authors:  Ming Chen; Chao Shi; Jing Zhao; Ziqing Gao; Chunzhi Zhang
Journal:  World J Microbiol Biotechnol       Date:  2016-08-26       Impact factor: 3.312
  2 in total

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