Literature DB >> 7406863

A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.

K Rose, J D Priddle, R E Offord, M P Esnouf.   

Abstract

When a polypeptide containing gamma-carboxyglutamic acid is decarboxylated in 2H2O, residue of (gamma gamma-2H2)glutamic acid are formed. Subsequent proteolytic digestion produces peptides which contain at each site 2H2-substituted and unsubstituted glutamic acid in the same ratio as existed for gramma-carboxy-substitution. The peptides may be identified and this ratio determined by combined gas chromatography--mass spectrometry. We also discuss decarboxylation in 3H2O followed by amino-acid analysis and Edman degradation.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7406863      PMCID： PMC1162513          DOI： 10.1042/bj1870239

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Isolation and characterization of vitamin K-dependent region of bovine blood clotting factor X.

Authors:  J B Howard; G L Nelsestuen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

2.  A new carboxylation reaction. The vitamin K-dependent incorporation of H-14-CO3- into prothrombin.

Authors:  C T Esmon; J A Sadowski; J W Suttie
Journal:  J Biol Chem       Date:  1975-06-25       Impact factor: 5.157

3.  The separation and sequencing of permethylated peptides by mass spectrometry directly coupled to gas-liquid chromatography.

Authors:  J D Priddle; K Rose; R E Offord
Journal:  Biochem J       Date:  1976-09-01       Impact factor: 3.857

4.  Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.

Authors:  H R Morris; A Dell
Journal:  Biochem J       Date:  1976-03-01       Impact factor: 3.857

5.  A variant of prothrombin induced in cattle by prolonged administration of warfarin.

Authors:  C V Prowse; P Mattock; M P Esnouf; A M Russell
Journal:  Biochim Biophys Acta       Date:  1976-05-20

6.  [A suitable arginine derivative for mass spectrometry and gas chromatography].

Authors:  H Vetter-Diechtl; W Vetter; W Richter; K Biemann
Journal:  Experientia       Date:  1968-04-15

7.  The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.

Authors:  H C Thøgersen; T E Petersen; L Sottrup-Jensen; S Magnusson; H R Morris
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857

8.  Specific tritium labeling of gamma-carboxyglutamic acid in proteins.

Authors:  P V Hauschka
Journal:  Biochemistry       Date:  1979-10-30       Impact factor: 3.162

9.  The gamma-carboxy glutamic acid content of human and bovine prothrombin following warfarin treatment.

Authors:  M P Esnouf; C V Prowse
Journal:  Biochim Biophys Acta       Date:  1977-02-22

10.  [3H]diborane reduction of vitamin K-dependent calcium-binding proteins. Identification of a unique amino acid.

Authors:  T H Zytkovicz; G L Nelsestuen
Journal:  J Biol Chem       Date:  1975-04-25       Impact factor: 5.157
  10 in total
  6 in total

Review 1.  Fast atom bombardment mass spectrometric characterization of peptides.

Authors:  P R Das; B N Pramanik
Journal:  Mol Biotechnol       Date:  1998-04       Impact factor: 2.695

Review 2.  Gamma-carboxyglutamate-containing proteins and the vitamin K-dependent carboxylase.

Authors:  C Vermeer
Journal:  Biochem J       Date:  1990-03-15       Impact factor: 3.857

3.  Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level.

Authors:  K Rose; M G Simona; R E Offord
Journal:  Biochem J       Date:  1983-11-01       Impact factor: 3.857

4.  A new mass-spectrometric C-terminal sequencing technique finds a similarity between gamma-interferon and alpha 2-interferon and identifies a proteolytically clipped gamma-interferon that retains full antiviral activity.

Authors:  K Rose; M G Simona; R E Offord; C P Prior; B Otto; D R Thatcher
Journal:  Biochem J       Date:  1983-11-01       Impact factor: 3.857

Review 5.  Post-translational carboxylation of preprothrombin.

Authors:  B C Johnson
Journal:  Mol Cell Biochem       Date:  1981-08-11       Impact factor: 3.396

6.  Evidence for the vitamin K-dependent gamma-carboxylation of the first glutamic acid residue in peptide substrates containing a diglutamyl sequence.

Authors:  A I Burgess; M P Esnouf; K Rose; R E Offord
Journal:  Biochem J       Date:  1983-10-01       Impact factor: 3.857
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.