Literature DB >> 7390974

Purification and characterization of tubulin-tyrosine ligase from porcine brain.

H Murofushi.   

Abstract

A tubulin-tyrosine ligase was purified from porcine brains using DEAE-cellulose chromatography, Sepharose-sebacic acid hydrazide-ATP affinity chromatography and Sepharose-tubulin affinity chromatography. The purified enzyme migrated as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated to be 46,000. The apparent molecular weight was 37,000 on a high speed liquid chromatograph equipped with gel filtration columns. The pH optimum for the activity was around 8 and a second peak was observed at around 6.5 8.5 microM ATP or 30 microM tyrosine gave half-maximal activity. The purified enzyme catalyzed the tyrosination of the alpha subunit of tubulin in vitro.

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Year:  1980        PMID： 7390974     DOI： 10.1093/oxfordjournals.jbchem.a132828

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  24 in total

Review 1.  Tubulin-tyrosine ligase, a long-lasting enigma.

Authors:  C Erck; R Frank; J Wehland
Journal:  Neurochem Res       Date:  2000-01       Impact factor: 3.996

2.  Cloning of rat olfactory bulb tubulin tyrosine ligase cDNA: a dominant negative mutant and an antisense cDNA increase the proliferation rate of cells in culture.

Authors:  Carlos R Mas; Carlos O Arregui; Adrián Filiberti; Carlos E Argaraña; Héctor S Barra
Journal:  Neurochem Res       Date:  2002-11       Impact factor: 3.996

Review 3.  Back on track - on the role of the microtubule for kinesin motility and cellular function.

Authors:  Stefan Lakämper; Edgar Meyhöfer
Journal:  J Muscle Res Cell Motil       Date:  2006-02-02       Impact factor: 2.698

Review 4.  Posttranslational tyrosination/detyrosination of tubulin.

Authors:  H S Barra; C A Arce; C E Argaraña
Journal:  Mol Neurobiol       Date:  1988       Impact factor: 5.590

Review 5.  The chemical complexity of cellular microtubules: tubulin post-translational modification enzymes and their roles in tuning microtubule functions.

Authors:  Christopher P Garnham; Antonina Roll-Mecak
Journal:  Cytoskeleton (Hoboken)       Date:  2012-04-26

6.  The nucleotide sequence of rat alpha-tubulin: 3'-end characteristics, and evolutionary conservation.

Authors:  I Ginzburg; L Behar; D Givol; U Z Littauer
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

7.  Complete amino acid sequence of alpha-tubulin from porcine brain.

Authors:  H Ponstingl; E Krauhs; M Little; T Kempf
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

8.  Steady-state kinetic mechanism of bovine brain tubulin: tyrosine ligase.

Authors:  N L Deans; R D Allison; D L Purich
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

9.  Post-translational incorporation of the antiproliferative agent azatyrosine into the C-terminus of alpha-tubulin.

Authors:  Silvia A Purro; C Gastón Bisig; María A Contin; Héctor S Barra; Carlos A Arce
Journal:  Biochem J       Date:  2003-10-01       Impact factor: 3.857

10.  Axonal microtubules necessary for generation of sodium current in squid giant axons: I. Pharmacological study on sodium current and restoration of sodium current by microtubule proteins and 260K protein.

Authors:  G Matsumoto; M Ichikawa; A Tasaki; H Murofushi; H Sakai
Journal:  J Membr Biol       Date:  1984       Impact factor: 1.843
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