Early decay of human neutrophil chemotactic responsiveness following isolation from peripheral blood.

The data presented show an early decay of chemotaxis and random mobility, compared to phagocytic and metabolic activity in neutrophils kept at 4 degrees C and 37 degrees C. Evidence is presented which suggests that hydrogen peroxide is one of the contributing factors in the loss of neutrophil chemotactic properties. The early decay of neutrophil chemotactic responsiveness is discussed in terms of the auto-oxidative damage caused to the cell membrane by hydrogen peroxide.