Literature DB >> 7380167

A radioimmunoassay for quantitation of secretory component.

K Kunishi.   

Abstract

Secretory component (SC) isolated from human colostrum was radioiodinated with chloramin-T method and it was subjected to a radioimmunoassay for quantitation of SC. A monospecific rabbit anti-sera against human SC which could react with free SC as well as immunoglobulin bound SC was used for the assay. The present method can measure as low as 300 ng/ml of SC. The level (mean +/- 2 S.D.) of serum SC in healthy subjects was determined to be 31.3 +/- 7.2 micrograms/ml. Studies with Sephadex G-200 gel filtration demonstrated that SC in the normal sera existed mostly as a form of SC-IgA. In contrast, free SC was never detected in them.

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Year:  1980        PMID： 7380167     DOI： 10.1007/bf02774928

Source DB:  PubMed          Journal:  Gastroenterol Jpn        ISSN： 0435-1339


  19 in total

1.  A SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA.

Authors:  D S SCHALCH; M L PARKER
Journal:  Nature       Date:  1964-09-12       Impact factor: 49.962

2.  Studies on translocation of immunoglobulins across intestinal epithelium. II. Immunoelectron-microscopic localization of immunoglobulins and secretory component in human intestinal mucosa.

Authors:  W R Brown; Y Isobe; P K Nakane
Journal:  Gastroenterology       Date:  1976-12       Impact factor: 22.682

3.  Binding of secretory piece to polymeric IgA and IgM paraproteins in vitro.

Authors:  J Rádl; F Klein; P van den Berg; A M de Bruyn; W Hijmans
Journal:  Immunology       Date:  1971-05       Impact factor: 7.397

4.  Serum placental lactogen levels during pregnancy and in trophoblastic disease.

Authors:  N Samaan; S C Yen; H Friesen; O H Pearson
Journal:  J Clin Endocrinol Metab       Date:  1966-12       Impact factor: 5.958

5.  Noval subunit in secretory IgA.

Authors:  M S Halpern; M E Koshland
Journal:  Nature       Date:  1970-12-26       Impact factor: 49.962

6.  An extension of the nomenclature for immunoglobulins.

Authors:  R Asofski; R A Binaghi; G M Edelman; H C Goodman; J F Heremans; L Hood; E A Kabat; J Rejnek; D S Rowe; P A Small; Z Trnka
Journal:  Immunochemistry       Date:  1970-05

7.  Mucosal and glandular distribution of immunoglobulin components: differential localization of free and bound SC in secretory epithelial cells.

Authors:  P Brandtzaeg
Journal:  J Immunol       Date:  1974-04       Impact factor: 5.422

8.  Quantitation of exocrine IgA in human serum in health and disease.

Authors:  R A Thompson; P Asquith
Journal:  Clin Exp Immunol       Date:  1970-10       Impact factor: 5.732

9.  High levels of secretory IgA and free secretory component in the serum of rats with bile duct obstruction.

Authors:  I Lemaître-Coelho; G D Jackson; J P Vaerman
Journal:  J Exp Med       Date:  1978-03-01       Impact factor: 14.307

10.  The human secretory immunoglobulin system: immunohistoligical localization of gamma A, secretory "piece," and lactoferrin in normal human tissues.

Authors:  D R Tourville; R H Adler; J Bienenstock; T B Tomasi
Journal:  J Exp Med       Date:  1969-02-01       Impact factor: 14.307
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  3 in total

1.  Studies on secretory component in digestive disease. III. Levels of serum secretory component in digestive disease.

Authors:  K Kunishi
Journal:  Gastroenterol Jpn       Date:  1980

2.  Serum secretory component level in myeloma patients with disorders of the secretory surface.

Authors:  K Kunishi; T Arima; T Yasuhara; J Narumoto; K Suwaki; S Kita; H Nagashima
Journal:  Gastroenterol Jpn       Date:  1980-04

3.  Studies on secretory component in digestive disease. IV. Levels of serum secretory component in liver disease.

Authors:  K Kunishi; T Arima; T Yasuhara; J Narumoto; K Suwaki; H Nagashima
Journal:  Gastroenterol Jpn       Date:  1980
  3 in total

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