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Literature DB >> 736891

Isolation of heavy satellite deoxyribonucleic acid from sea-urchin spermatozoa.

Abstract

Two different methods were used to isolate high-molecular-weight heavy satellite DNA from the spermatozoa of the sea-urchin Lytechinus variegratus. Purification of satellite rDNA (ribosomal DNA) by selective denaturation of the spermatozoal DNA followed by a separation of the native and denatured DNA in an aqueous mixture of poly(ethylene glycol)/dextran is affected by the molecular weight of the spermatozoal DNA. Enrichment of satellite rDNA by selective precipitation of main-band DNA by poly-L-lysine is cumbersome but more suitable.

Entities: Chemical Species

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Year: 1978 PMID： 736891 PMCID： PMC1186035 DOI： 10.1042/bj1750015

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

Review 4. Methods of gene isolation.

Authors: D D Brown; R Stern
Journal: Annu Rev Biochem Date: 1974 Impact factor: 23.643

Review 5. Highly repetitive sequences of DNA in chromosomes.

Authors: W G Flamm
Journal: Int Rev Cytol Date: 1972

6. Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.

Authors: B M Alberts
Journal: Biochemistry Date: 1967-08 Impact factor: 3.162

7. DNA circularization: a molecular approach for the detection and isolation of tandemly duplicated genes.

Authors: L Polito; F Graziani; E Boncinelli; C Malva; F Ritossa
Journal: Nat New Biol Date: 1971-01-20

Review 8. The ribosomal RNA cistrons.

Authors: M L Birnstiel; M Chipchase; J Speirs
Journal: Prog Nucleic Acid Res Mol Biol Date: 1971

Isolation of heavy satellite deoxyribonucleic acid from sea-urchin spermatozoa.

1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

2. On the inactivation of transforming DNA by temperatures below the melting point.

3. Mechanisms of inactivation of deoxyribonucleic acids by heat.

Review 4. Methods of gene isolation.

Review 5. Highly repetitive sequences of DNA in chromosomes.

6. Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.

7. DNA circularization: a molecular approach for the detection and isolation of tandemly duplicated genes.

Review 8. The ribosomal RNA cistrons.

Review 9. "Repetitive" DNA in higher organisms.

10. The formation apurinic acid from the desoxyribonucleic acid of calf thymus.

1. Study of ribosomal deoxyribonucleic acid of sea urchin.

2. Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm.

3. Intragenomic variation in ribosomal RNA gene of the sea urchin Lytechinus variegatus.