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Literature DB >> 488263

Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm.

Abstract

Electron microscopy of the partially heat denatured ribosomal DNA (rDNA) from sea urchin (Lytechinus variegatus) sperm has demonstrated that it consists of repeating units of 3.6 +/- 0.2 micron, corresponding to a mol.wt of 7.2 +/- 0.4 x 10(6). Based on differential denaturability, each repeat unit is divided into 2 regions. The larger region of 2.47 +/- 0.11 micron (mol.wt 4.9 +/- 0.22 x 10(6)) corresponds in length to the ribosomal precursor RNA of sea urchins and the smaller, GC-rich, subunit of 1.16 +/- 0.09 micrometer (mol.wt 2.3 +/- 0.18 x 10(6)) is presumed to contain non-transcribed spacer sequences.

Entities: Disease Species

Mesh：

Substances：
DNA

Year: 1979 PMID： 488263 DOI： 10.1007/bf01963262

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

24 in total

1. [Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages].

Authors: A K KLEINSCHMIDT; D LANG; D JACHERTS; R K ZAHN
Journal: Biochim Biophys Acta Date: 1962-12-31

2. Sea urchin satellite deoxyribonucleic acid. Its large-scale isolation and hybridization with homologous ribosomal ribonucleic acid.

Authors: J B Patterson; D W Stafford
Journal: Biochemistry Date: 1970-03-03 Impact factor: 3.162

3. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.

Authors: J H Sinclair; D D Brown
Journal: Biochemistry Date: 1971-07-06 Impact factor: 3.162

4. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis.

Authors: M Birnstiel; J Speirs; I Purdom; K Jones; U E Loening
Journal: Nature Date: 1968-08-03 Impact factor: 49.962

5. Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA.

Authors: D D Brown; C S Weber
Journal: J Mol Biol Date: 1968-06-28 Impact factor: 5.469

Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm.

1. [Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages].

2. Sea urchin satellite deoxyribonucleic acid. Its large-scale isolation and hybridization with homologous ribosomal ribonucleic acid.

3. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.

4. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis.

5. Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA.

6. Satellite DNA (II) from sea urchin (Lytechinus variegatus) sperm.

7. Localization of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis.

8. The structural organization of ribosomal DNA in Drosophila melanogaster.

9. A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes.

10. Observations on early germ cell development and premeiotic ribosomal DNA amplification in Xenopus laevis.

1. Intragenomic variation in ribosomal RNA gene of the sea urchin Lytechinus variegatus.