Literature DB >> 7335497

(E)-5-(2-bromovinyl)-2'-deoxyuridine-5'-triphosphate as a DNA polymerase substrate.

J Sági, A Szabolcs, A Szemzö, L Otvös.   

Abstract

Time course of incorporation and the effect of 5'-triphosphate of the selective antiherpetic agent (E)-5-(2-bromovinyl)-2'-deoxyuridine (bv5dUTP) on the incorporation of dTTP and dATP into template-primers of different structure were studied in E. coli DNA polymerase I Klenow fragment enzyme-catalyzed reactions. bv5dUTP could substitute for dTTP depending on the structure of template-primer. E.g. into calf thymus DNA incorporation of bv5dUMP was around 80% of that of dTMP at 30 minutes of incubation. The analog has also inhibited dTMP incorporation, net DNA synthesis, however, was hardly affected. The substrate properties of the analog were studied with [2-14C]-labelled bv5dUTP.

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Year:  1981        PMID： 7335497      PMCID： PMC327656          DOI： 10.1093/nar/9.24.6985

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

Authors:  H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1962-02       Impact factor: 5.157

Review 2.  Antiviral and antitumor activities of 5-substituted 2'-deoxyuridines.

Authors:  E De Clercq
Journal:  Methods Find Exp Clin Pharmacol       Date:  1980-10

3.  Polymorphism of DNA double helices.

Authors:  A G Leslie; S Arnott; R Chandrasekaran; R L Ratliff
Journal:  J Mol Biol       Date:  1980-10-15       Impact factor: 5.469

4.  Oral (E)-5-(2-bromovinyl)-2'-deoxyuridine in severe herpes zoster.

Authors:  E de Clercq; H Degreef; J Wildiers; G de Jonge; A Drochmans; J Descamps; P de Somer
Journal:  Br Med J       Date:  1980-11-01

5.  (E)-5-(2-Bromovinyl)-2'-deoxyuridine: a potent and selective anti-herpes agent.

Authors:  E De Clercq; J Descamps; P De Somer; P J Barr; A S Jones; R T Walker
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

6.  A study of substrate specificity of mammalian and bacterial DNA polymerases with 5-alkyl-2'-deoxyuridine 5'-triphosphates.

Authors:  J Sági; R Nowak; B Zmudzka; A Szemzö; L Otvös
Journal:  Biochim Biophys Acta       Date:  1980-02-29

7.  Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.

Authors:  J T Sági; A Szabolcs; A Szemzö; L Otvös
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

8.  Efficacy of (E)-5-(2-bromovinyl)-2'-deoxyuridine in the topical treatment of herpes simplex keratitis.

Authors:  P C Maudgal; L Missotten; E De Clercq; J Descamps; E De Meuter
Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol       Date:  1981

9.  On the mechanism of selective inhibition of herpesvirus replication by (E)-5-(2-bromovinyl)-2'-deoxyuridine.

Authors:  H S Allaudeen; J W Kozarich; J R Bertino; E De Clercq
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205
  9 in total
  3 in total

1.  Crystal and molecular structure of (E)-5-(2-bromovinyl-2'-deoxyuridine).

Authors:  L Párkányi; A Kálmán; T Kovács; A Szabolcs; L Otvös
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

2.  Modified polynucleotides. VII. Impaired integrity of a synthetic DNA containing the antiherpetic agent 5-isopropyl-2'-deoxyuridine.

Authors:  J Sági; A Szabolcs; A Szemźó; L Otvös
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

3.  Modified polynucleotides. VI. Properties of a synthetic DNA containing the anti-herpes agent (E)-5-(2-bromovinyl)-2'-deoxyuridine.

Authors:  J Sági; A Czuppon; M Kajtár; A Szabolcs; A Szemzö; L Otvös
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971
  3 in total

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