Literature DB >> 6755397

Modified polynucleotides. VI. Properties of a synthetic DNA containing the anti-herpes agent (E)-5-(2-bromovinyl)-2'-deoxyuridine.

J Sági, A Czuppon, M Kajtár, A Szabolcs, A Szemzö, L Otvös.   

Abstract

A new modified polydeoxynucleotide, a copolymer of nucleotides of 2'-deoxyadenosine and the very efficacious anti-herpesvirus agent (E)-5-(2-bromovinyl)-2'-deoxyuridine was synthesized with E. coli DNA polymerase I enzyme. It is characterized by its physical (absorption and circular dichroism spectra, thermal transition, sedimentation analysis) and bioorganic (template activity, stability) properties. Compared to poly [d(A-T)], the modified polydeoxynucleotide had a lower thermal stability but exhibited higher stability against DNases and higher template activity for DNA synthesis. Template activity for RNA synthesis of this template was, however, poor and extent of AMP and UMP incorporation was limited as well.

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Year:  1982        PMID： 6755397      PMCID： PMC320950          DOI： 10.1093/nar/10.19.6051

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Mercurated polynucleotides: new probes for hybridization and selective polymer fractionation.

Authors:  R M Dale; D C Ward
Journal:  Biochemistry       Date:  1975-06-03       Impact factor: 3.162

2.  "Aromatic" substituent constants for structure-activity correlations.

Authors:  C Hansch; A Leo; S H Unger; K H Kim; D Nikaitani; E J Lien
Journal:  J Med Chem       Date:  1973-11       Impact factor: 7.446

3.  Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences.

Authors:  R D Wells; J E Larson; R C Grant; B E Shortle; C R Cantor
Journal:  J Mol Biol       Date:  1970-12-28       Impact factor: 5.469

4.  Specificity of the three-stranded complex formation between double-stranded DNA and single-stranded RNA containing repeating nucleotide sequences.

Authors:  A R Morgan; R D Wells
Journal:  J Mol Biol       Date:  1968-10-14       Impact factor: 5.469

5.  Comparative properties of DNA, RNA, and hybrid homopolymer pairs.

Authors:  M J Chamberlin
Journal:  Fed Proc       Date:  1965 Nov-Dec

6.  Circular dichroism of adenine and thymine containing synthetic polynucleotides.

Authors:  J Greve; M F Maestre; A Levin
Journal:  Biopolymers       Date:  1977-07       Impact factor: 2.505

7.  Conformations and structural transitions in polydeoxynucleotides.

Authors:  J Pilet; J Blicharski; J Brahms
Journal:  Biochemistry       Date:  1975-05-06       Impact factor: 3.162

8.  Effect of mercuric and silver ions on cesium sulfate equilibrium buoyant densities of synthetic polydeoxyribonucleotides.

Authors:  M W Lieberman; D J Harvan; D E Amacher; J B Patterson
Journal:  Biochim Biophys Acta       Date:  1976-03-17

9.  Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.

Authors:  J T Sági; A Szabolcs; A Szemzö; L Otvös
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

10.  The synthesis and enzymatic polymerization of nucleotides containing mercury: potential tools for nucleic acid sequencing and structural analysis.

Authors:  R M Dale; D C Livingston; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205
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  6 in total

1.  Minimising the secondary structure of DNA targets by incorporation of a modified deoxynucleoside: implications for nucleic acid analysis by hybridisation.

Authors:  H K Nguyen; E M Southern
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

2.  C4-methyldeoxythymidine replacing deoxythymidine in poly[d(A-T)] renders the polymer resistant to the 3'----5' exonuclease activity of the Klenow and T4 DNA polymerases.

Authors:  B Singer
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

Review 3.  Structure-activity relationships and conformational features of antiherpetic pyrimidine and purine nucleoside analogues. A review.

Authors:  T Kulikowski
Journal:  Pharm World Sci       Date:  1994-04-15

4.  A comparative study of the thermal stability of oligodeoxyribonucleotides containing 5-substituted 2'-deoxyuridines.

Authors:  M Ahmadian; P Zhang; D E Bergstrom
Journal:  Nucleic Acids Res       Date:  1998-07-01       Impact factor: 16.971

5.  Modified polynucleotides. VII. Impaired integrity of a synthetic DNA containing the antiherpetic agent 5-isopropyl-2'-deoxyuridine.

Authors:  J Sági; A Szabolcs; A Szemźó; L Otvös
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

6.  Uracil in deoxyribonucleotide polymers reduces their template-primer activity for E. coli DNA polymerase I.

Authors:  J A Vilpo; J Ridell
Journal:  Nucleic Acids Res       Date:  1983-06-11       Impact factor: 16.971
  6 in total

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