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Literature DB >> 7332527

Genetic variability of proteins from plasma membranes and cytosols of mouse organs.

Abstract

The genetic variability of membrane proteins (structure-bound proteins) and cytosol proteins (water-soluble proteins) was investigated in two inbred strains of the mouse, C57BL/6J and DBA/2J. Membrane proteins and cytosol were isolated from the brain and liver of the mouse. The proteins were separated by two-dimensional electrophoresis. A high number of genetic variant proteins (brain, 30; liver, 72) was found in the cytosol. Most of these variants represented changes in the amount of proteins. Electrophoretic mobility changes occurred only in about 1% (brain, 6; liver, 9) of all protein spots of a two-dimensional pattern. In contrast to the cytosol proteins, no genetic variation was detected among the membrane proteins, not even for the quantitative characteristics of the protein spots. The results obtained for the two classes of proteins suggest that the degree of variability in the amount of proteins is related to the degree of variability in the structure of proteins.

Entities: Chemical Species

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Year: 1981 PMID： 7332527 DOI： 10.1007/bf00504251

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

22 in total

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Authors: W E Bucknall; H H Kazazian; L T Clement; B Childs
Journal: Johns Hopkins Med J Date: 1975-09

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Authors: E Zuckerkandl
Journal: J Mol Evol Date: 1976-04-09 Impact factor: 2.395

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Authors: L Anderson; N G Anderson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

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Authors: N L Anderson; N G Anderson
Journal: Biochem Biophys Res Commun Date: 1979-05-14 Impact factor: 3.575

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Authors: T K Ray
Journal: Biochim Biophys Acta Date: 1970-01-06

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Authors: R J Britten; E H Davidson
Journal: Science Date: 1969-07-25 Impact factor: 47.728

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Authors: J Klose
Journal: Humangenetik Date: 1975

8. Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs.

Authors: J Klose
Journal: J Mol Evol Date: 1982 Impact factor: 2.395

9. Calf and pea histone IV. 3. Complete amino acid sequence of pea seedling histone IV; comparison with the homologous calf thymus histone.

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Journal: J Biol Chem Date: 1969-10-25 Impact factor: 5.157

10. Isoelectric focusing and electrophoresis combined as a method for defining new point mutations in the mouse.

Authors: J Klose
Journal: Genetics Date: 1979-05 Impact factor: 4.562

15 in total

1. Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize.

Authors: C Damerval; Y Hébert; D de Vienne
Journal: Theor Appl Genet Date: 1987-06 Impact factor: 5.699

2. Differing amounts of genetic polymorphism in testes and male accessory glands of Drosophila melanogaster and Drosophila simulans.

Authors: M B Coulthart; R S Singh
Journal: Biochem Genet Date: 1988-02 Impact factor: 1.890

3. Genetic variation in proteins: comparison of one-dimensional and two-dimensional gel electrophoresis.

Authors: T McLellan; G F Ames; K Nikaido
Journal: Genetics Date: 1983-06 Impact factor: 4.562

4. Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs.

Authors: J Klose
Journal: J Mol Evol Date: 1982 Impact factor: 2.395

5. Analysis of two-dimensional protein patterns from mouse embryos with different trisomies.

Authors: J Klose; B Putz
Journal: Proc Natl Acad Sci U S A Date: 1983-06 Impact factor: 11.205

6. Genetic variability of proteins from mitochondria and mitochondrial fractions of mouse organs.

Authors: P Jungblut; J Klose
Journal: Biochem Genet Date: 1985-04 Impact factor: 1.890

7. Two-dimensional electrophoresis of soluble and structure-bound proteins from cultured human fibroblasts and hair root cells: qualitative and quantitative variation.

Authors: J Klose; I Willers; S Singh; H W Goedde
Journal: Hum Genet Date: 1983 Impact factor: 4.132

8. Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 3. Frequent occurrence of genetic variants in some abundant polypeptides of PHA-stimulated peripheral blood lymphocytes.

Authors: H Hamaguchi; M Yamada; M Shibasaki; R Mukai; T Yabe; I Kondo
Journal: Hum Genet Date: 1982 Impact factor: 4.132

9. Mice homozygous for chromosomal deletions at the albino locus region lack specific polypeptides in two-dimensional gels.

Authors: L J Baier; S M Hanash; R P Erickson
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

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Authors: G S Spicer
Journal: J Mol Evol Date: 1988 Impact factor: 2.395