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Literature DB >> 7325970

Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield.

Abstract

Cathepsin D was purified by two-step affinity chromatography on concanavalin A-- and pepstatin--Sepharose. The main purification was achieved by washing the enzyme bound to the pepstatin--Sepharose column with buffered 6 M-urea. This step separated cathepsin D from all low- and high-molecular-weight impurities. Although the 1700-fold purified acid proteinase was homogeneous on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, it still showed microheterogeneity.

Entities: Chemical Gene Species

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Year: 1981 PMID： 7325970 PMCID： PMC1163155 DOI： 10.1042/bj1970519

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. [Intracellular protein breakdown. VI. Isolation, properties and biological significance of cathepsin D from rat liver].

Authors: B Wiederanders; S Ansorge; P Bohley; U Broghammer; H Kirschke; J Langner
Journal: Acta Biol Med Ger Date: 1976

Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield.

1. [Intracellular protein breakdown. VI. Isolation, properties and biological significance of cathepsin D from rat liver].

2. Protein measurement with the Folin phenol reagent.

3. Cathepsin D: rapid isolation by affinity chromatography on haemoglobin-agarose resin.

4. Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose.

5. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

6. Proteinase and proteinase-inhibitor activities of rat uterine myometrium during pregnancy and involution.

7. Maturation of the head of bacteriophage T4. I. DNA packaging events.

8. Hydrophobic chromatography.

9. Early events in the biosynthesis of the lysosomal enzyme cathepsin D.

10. Cathepsin D isozymes from porcine spleens. Large scale purification and polypeptide chain arrangements.

1. Prolactin crinophagy is induced in the estrogen-stimulated male rat pituitary.

2. The effects of lactoyl-pepstatin and the pepsin inhibitor peptide on pig cathepsin D.

3. Immunolocalization of cathepsin D in normal and neoplastic human tissues.

4. A systematic series of synthetic chromophoric substrates for aspartic proteinases.

5. Cathepsin D from pig myometrium. Characterization of the proteinase.

6. Prognostic significance of cathepsin-D in patients with breast cancer.