Literature DB >> 7309847

Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.

W Engels, M A Kamps, C P van Boven.   

Abstract

A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specific, quantitative, and easily automated. If offers clinical laboratories a more reliable assay of staphylocoagulase and is a valuable aid for the rapid identification of S. aureus.

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Year:  1981        PMID： 7309847      PMCID： PMC273976          DOI： 10.1128/jcm.14.5.496-500.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Interpretation of the tube coagulase test for identification of Staphylococcus aureus.

Authors:  W H Sperber; S R Tatini
Journal:  Appl Microbiol       Date:  1975-04

2.  Isolation and identification of Staphylococcus hyicus.

Authors:  L A Devriese
Journal:  Am J Vet Res       Date:  1977-06       Impact factor: 1.156

3.  Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.

Authors:  W Engels; M Kamps; C P van Boven
Journal:  J Gen Microbiol       Date:  1978-12

4.  A solid medium for visual demonstration of coagulase production by Staphylococcus aureus.

Authors:  J C van der Vijver; C A Kraayeveld; M F Michel
Journal:  J Clin Pathol       Date:  1972-05       Impact factor: 3.411

5.  An automated system for the quantitative determination of proteolytic enzymes using azocasein.

Authors:  G G Hazen; J A Hause; J A Hubicki
Journal:  Ann N Y Acad Sci       Date:  1965-11-09       Impact factor: 5.691

6.  Pseudocoagulase activity of staphylococci.

Authors:  Z Wegrzynowicz; P B Heczko; J Jeljaszewicz; M Neugebauer; G Pulverer
Journal:  J Clin Microbiol       Date:  1979-01       Impact factor: 5.948

7.  On the nature of albumin-promoted coagulase release by Staphylococcus aureus.

Authors:  R A Altenbern
Journal:  J Infect Dis       Date:  1966-12       Impact factor: 5.226

8.  Activation of a pro-enzyme by a stoichiometric reaction with another protein. The reaction between prothrombin and staphylocoagulase.

Authors:  H C Hemker; B M Bas; A D Muller
Journal:  Biochim Biophys Acta       Date:  1975-01-30

9.  Pour-plate method for the detection of coagulase production by Staphylococcus aureus.

Authors:  J T Parisi; J N Baldwin; M Sottile
Journal:  Appl Microbiol       Date:  1973-04

10.  Continuous culture studies on the production of staphylocoagulase by Staphylococcus aureus.

Authors:  W Engels; M A Kamps; C P van Boven
Journal:  Antonie Van Leeuwenhoek       Date:  1980       Impact factor: 2.271
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  6 in total

1.  Comparison of the affinities to bovine and human prothrombin of the staphylocoagulases from Staphylococcus intermedius and Staphylococcus aureus of animal origin.

Authors:  J Raus; D N Love
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

Review 2.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

Review 3.  Use of enzyme tests in characterization and identification of aerobic and facultatively anaerobic gram-positive cocci.

Authors:  S Bascomb; M Manafi
Journal:  Clin Microbiol Rev       Date:  1998-04       Impact factor: 26.132

4.  Comparison of the staphylocoagulase activities of Staphylococcus aureus and Staphylococcus intermedius on Chromozym-TH.

Authors:  J Raus; D N Love
Journal:  J Clin Microbiol       Date:  1990-02       Impact factor: 5.948

5.  Characterization of coagulase-positive Staphylococcus intermedius and Staphylococcus aureus isolated from veterinary clinical specimens.

Authors:  J Raus; D N Love
Journal:  J Clin Microbiol       Date:  1983-10       Impact factor: 5.948

6.  Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays.

Authors:  M G Holliday; M Ford; J D Perry; F K Gould
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948
  6 in total

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