| Literature DB >> 7306952 |
M Hartwig, E Matthes, W Arnold.
Abstract
The superhelical properties of chromosomal DNA from cells of a mouse sarcoma were investigated in neutral sucrose gradients containing ethidium bromide. Removal of negative supercoiling from the DNA of the sarcoma cells required a substantially higher dye concentration than was necessary in the case of DNA from cultured mouse fibroblasts. The calculated value of the mean superhelical density in malignant cells (sigma = -0.14) appears abnormally high compared with the value (sigma = -0.09) obtained for DNA of mouse fibroblasts. Chromosomal DNA from mouse sarcoma cells is therefore concluded to be highly deficient in helical turns.Entities:
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Year: 1981 PMID： 7306952 DOI： 10.1016/0304-3835(81)90142-7
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679