Literature DB >> 7295541

Some cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes. I. A simple method for isolation of hepatocyte nuclei using in situ perfusion of the liver.

P R Steele, A P Yim, B M Herbertson, J Watson.   

Abstract

A simple method for isolating mouse hepatocyte nuclei is presented, which requires no special apparatus. Flow cytofluorimetric scans of hepatocyte nuclei, prepared by this method and stained with ethidium bromide, showed that adult mice have a high proportion of their hepatocyte nuclei in the tetraploid and octaploid states. Polyploidy was more pronounced in female animals.

Entities:  

Mesh：

Year:  1981        PMID： 7295541      PMCID： PMC2041712     

Source DB:  PubMed          Journal:  Br J Exp Pathol        ISSN： 0007-1021


  3 in total

1.  Cell size, nuclear content, and the development of polyploidy in the Mammalian liver.

Authors:  C J Epstein
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

2.  Cell microfluorometry: a method for rapid fluorescence measurement.

Authors:  M A Van Dilla; T T Trujillo; P F Mullaney; J R Coulter
Journal:  Science       Date:  1969-03-14       Impact factor: 47.728

3.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

Authors:  A Krishan
Journal:  J Cell Biol       Date:  1975-07       Impact factor: 10.539
  3 in total
  5 in total

1.  Flow cytometric analysis of mouse hepatocyte ploidy. I. Preparative and mathematical protocol.

Authors:  E Severin; E M Meier; R Willers
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

2.  Some flow-cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes: iii. further observations on early changes in nuclear ploidy of mouse hepatocytes following various experimental procedures.

Authors:  A P Yim
Journal:  Br J Exp Pathol       Date:  1982-08

3.  Some flow cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes. II. Early changes in nuclear ploidy of mouse hepatocytes following carbon tetrachloride administration: evidence for polyploid nuclei arrested in telophase.

Authors:  P R Steele; A P Yim; B M Herbertson; J Watson
Journal:  Br J Exp Pathol       Date:  1981-10

4.  Multiparameter flow cytometric analysis of hepatic nuclear RNA and DNA of normal and hepatotoxin-treated mice.

Authors:  P J Higgins; M Piwnicka; Z Darzynkiewicz; M R Melamed
Journal:  Am J Pathol       Date:  1984-04       Impact factor: 4.307

5.  Accumulation of abnormally high ploid nuclei in the liver of LEC rats developing spontaneous hepatitis.

Authors:  Y Fujimoto; M Oyamada; A Hattori; H Takahashi; M Sawaki; K Dempo; M Mori; M Nagao
Journal:  Jpn J Cancer Res       Date:  1989-01
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.