Literature DB >> 728995

Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

I Blikstad, F Markey, L Carlsson, T Persson, U Lindberg.   

Abstract

A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.

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Year:  1978        PMID： 728995     DOI： 10.1016/0092-8674(78)90277-5

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  118 in total

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