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Literature DB >> 7279659

Construction of bacterial plasmids containing sequences complementary to chicken alpha-tropomyosin mRNA.

Abstract

Recombinant plasmids have been constructed with contain sequences complementary to the mRNA coding for skeletal muscle alpha-tropomyosin. These recombinants were detected initially using a selective cDNA probe and subsequently using a messenger RNA selection assay. alpha-TM plasmids hybridize to a singly mRNA species smaller than 18S ribosomal RNA and found only in skeletal muscle. Cross-hybridization with mRNA's coding for other tropomyosins could not be detected under normal conditions. However, under conditions of reduced stringency alpha- TM plasmids cross-hydridize with an RNA species in heart muscle which may code for cardiac tropomyosin.

Entities: Gene Species

Mesh：

Substances：

Year: 1981 PMID： 7279659 PMCID： PMC326884 DOI： 10.1093/nar/9.12.2675

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

34 in total

1. DNA nucleotide sequence restricted by the RI endonuclease.

Authors: J Hedgpeth; H M Goodman; H W Boyer
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

2. The subunits and biological activity of polymorphic forms of tropomyosin.

Authors: P Cummins; S V Perry
Journal: Biochem J Date: 1973-08 Impact factor: 3.857

3. Tropomyosin in brain and growing neurones.

Authors: R E Fine; A L Blitz; S E Hitchcock; B Kaminer
Journal: Nat New Biol Date: 1973-10-10

4. Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine.

Authors: S K Shapiro; D J Ehninger
Journal: Anal Biochem Date: 1966-05 Impact factor: 3.365

5. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

6. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

Authors: V M Vogt
Journal: Eur J Biochem Date: 1973-02-15

7. A tropomyosin-like protein from human platelets.

Authors: I Cohen; C Cohen
Journal: J Mol Biol Date: 1972-07-21 Impact factor: 5.469

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

9. Purification and properties of the replicative intermediate of the RNA bacteriophage R17.

Authors: R M Franklin
Journal: Proc Natl Acad Sci U S A Date: 1966-06 Impact factor: 11.205

10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors: H Aviv; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

7 in total

1. The mRNA and RNA-copy pseudogenes encoding TM30nm, a human cytoskeletal tropomyosin.

Authors: A R MacLeod; C Houlker; F C Reinach; K Talbot
Journal: Nucleic Acids Res Date: 1986-11-11 Impact factor: 16.971

2. Analysis of tropomyosin cDNAs isolated from skeletal and smooth muscle mRNA.

Authors: J Flach; G Lindquester; S Berish; K Hickman; R Devlin
Journal: Nucleic Acids Res Date: 1986-11-25 Impact factor: 16.971

3. Multiple tropomyosin polypeptides in chicken embryo fibroblasts: differential repression of transcription by Rous sarcoma virus transformation.

Authors: M Hendricks; H Weintraub
Journal: Mol Cell Biol Date: 1984-09 Impact factor: 4.272