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Literature DB >> 72759

Enzyme-linked immunosorbent assay for chlamydial antibodies.

Abstract

An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The assay antigen produced in cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by ELISA. Paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by ELISA also. Control sera from persons with no history of chlamydial infection were negative by both tests. Sera from patients with psittacosis or lymphogranuloma venereum were ELISA positive, indicating that the assay with the antigen used in this study is genus specific rather than species specific.

Entities: Chemical Disease Species

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Year: 1977 PMID： 72759 PMCID： PMC274806 DOI： 10.1128/jcm.6.5.507-510.1977

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

19 in total

1. Amino acid metabolism in mammalian cell cultures.

Authors: H EAGLE
Journal: Science Date: 1959-08-21 Impact factor: 47.728

2. A passive hemagglutination reaction for psittacosis.

Authors: A A BENEDICT; E O'BRIEN
Journal: J Immunol Date: 1958-02 Impact factor: 5.422

3. A microplate method of enzyme-linked immunosorbent assay and its application to malaria.

Authors: A Voller; D Bidwell; G Huldt; E Engvall
Journal: Bull World Health Organ Date: 1974 Impact factor: 9.408

4. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors: E Engvall; P Perlmann
Journal: J Immunol Date: 1972-07 Impact factor: 5.422

5. Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody.

Authors: S P Wang; J T Grayston; E R Alexander; K K Holmes
Journal: J Clin Microbiol Date: 1975-03 Impact factor: 5.948

6. Enzyme-linked immunosorbent assays for cholera serology.

Authors: J Holmgren; A M Svennerholm
Journal: Infect Immun Date: 1973-05 Impact factor: 3.441

7. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.

Authors: A Voller; D E Bidwell
Journal: Br J Exp Pathol Date: 1976-04

8. Chlamydial infection in women with cervical dysplasia.

Authors: J Schachter; E C Hill; E B King; V R Coleman; P Jones; K F Meyer
Journal: Am J Obstet Gynecol Date: 1975-12-01 Impact factor: 8.661

9. A simple method for detecting antibodies to rubella.

Authors: A Voller; D E Bidwell
Journal: Br J Exp Pathol Date: 1975-08

10. FLUORESCENT CELL-COUNTING NEUTRALIZATION TEST FOR PSITTACOSIS.

Authors: N HAHON; K O COOKE
Journal: J Bacteriol Date: 1965-06 Impact factor: 3.490

23 in total

1. Enzyme-linked immunosorbent assay using three different antigen preparations for detection of antibodies to Chlamydia trachomatis.

Authors: J Raymond; P Duc-Goiran; S Joundy; J Orfila; J Acar
Journal: Eur J Clin Microbiol Date: 1985-10 Impact factor: 3.267

9. Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

Authors: K Granfors
Journal: J Clin Microbiol Date: 1979-03 Impact factor: 5.948

10. The development and evaluation of a mu-capture ELISA detecting Chlamydia-specific IgM.

Authors: T G Wreghitt; V J Robinson; E O Caul; I D Paul; S Gatley
Journal: Epidemiol Infect Date: 1988-10 Impact factor: 2.451

Enzyme-linked immunosorbent assay for chlamydial antibodies.

1. Amino acid metabolism in mammalian cell cultures.

2. A passive hemagglutination reaction for psittacosis.

3. A microplate method of enzyme-linked immunosorbent assay and its application to malaria.

4. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

5. Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody.

6. Enzyme-linked immunosorbent assays for cholera serology.

7. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.

8. Chlamydial infection in women with cervical dysplasia.

9. A simple method for detecting antibodies to rubella.

10. FLUORESCENT CELL-COUNTING NEUTRALIZATION TEST FOR PSITTACOSIS.

1. Enzyme-linked immunosorbent assay using three different antigen preparations for detection of antibodies to Chlamydia trachomatis.

2. Use of the enzyme-linked immunosorbent assay for detection of antibodies to Chlamydia trachomatis.

3. Comparison of four serological methods for detecting antibodies to Chlamydia trachomatis.

4. Enzyme-linked immunosorbent assay for serodiagnosis of Mycoplasma pneumoniae infections.

5. Enzyme-linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies.

6. Enzyme-linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera.

7. Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.

8. Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults.

9. Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

10. The development and evaluation of a mu-capture ELISA detecting Chlamydia-specific IgM.