Literature DB >> 6863503

Enzyme-linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera.

M P Finn, A Ohlin, J Schachter.   

Abstract

Microimmunofluorescence methods used for detection of immunoglobulin G and M antibodies to Chlamydia trachomatis are not available to many clinical laboratories. We evaluated a simple enzyme-linked immunosorbent assay in which serotype L2 elementary bodies are used as antigen. The enzyme-linked immunosorbent assay proved satisfactory for the detection of serum IgG. A total of 160 human sera were tested, and the results correlated well with those obtained by microimmunofluorescence. Results for IgM antibody detection were not as successful, and correlation with current methods was poor.

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Year:  1983        PMID： 6863503      PMCID： PMC272753          DOI： 10.1128/jcm.17.5.848-852.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.

Authors:  K O Kalimo; O H Meurman; P E Halonen; B R Ziola; M K Viljanen; K Granfors; P Toivanen
Journal:  J Clin Microbiol       Date:  1976-08       Impact factor: 5.948

Review 3.  Chlamydial infections (third of three parts).

Authors:  J Schachter
Journal:  N Engl J Med       Date:  1978-03-09       Impact factor: 91.245

4.  Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test.

Authors:  S P Wang; J T Grayston
Journal:  Am J Ophthalmol       Date:  1970-09       Impact factor: 5.258

5.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

6.  Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A.

Authors:  W Duermeyer; J van der Veen
Journal:  Lancet       Date:  1978-09-23       Impact factor: 79.321

Review 7.  Chlamydial infections.

Authors:  J Schachter; M Grossman
Journal:  Annu Rev Med       Date:  1981       Impact factor: 13.739

8.  Enzyme-linked immunosorbent assay for chlamydial antibodies.

Authors:  V J Lewis; W L Thacker; S H Mitchell
Journal:  J Clin Microbiol       Date:  1977-11       Impact factor: 5.948

9.  Serology of Chlamydia trachomatis in infants.

Authors:  J Schachter; M Grossman; P H Azimi
Journal:  J Infect Dis       Date:  1982-10       Impact factor: 5.226

10.  Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis.

Authors:  H D Caldwell; J Kromhout; J Schachter
Journal:  Infect Immun       Date:  1981-03       Impact factor: 3.441
  10 in total
  11 in total

1.  Effect of a three month course of ciprofloxacin on the outcome of reactive arthritis.

Authors:  T Yli-Kerttula; R Luukkainen; U Yli-Kerttula; T Möttönen; M Hakola; M Korpela; M Sanila; J Parviainen; J Uksila; R Vainionpää; A Toivanen
Journal:  Ann Rheum Dis       Date:  2000-07       Impact factor: 19.103

2.  Tubal infertility in the Gambia: chlamydial and gonococcal serology in women with tubal occlusion compared with pregnant controls.

Authors:  D C Mabey; G Ogbaselassie; J N Robertson; J E Heckels; M E Ward
Journal:  Bull World Health Organ       Date:  1985       Impact factor: 9.408

3.  Enzyme-linked immunosorbent assay using three different antigen preparations for detection of antibodies to Chlamydia trachomatis.

Authors:  J Raymond; P Duc-Goiran; S Joundy; J Orfila; J Acar
Journal:  Eur J Clin Microbiol       Date:  1985-10       Impact factor: 3.267

4.  Bacterial antibodies in ankylosing spondylitis.

Authors:  O Mäki-Ikola; K Lehtinen; K Granfors; R Vainionpää; P Toivanen
Journal:  Clin Exp Immunol       Date:  1991-06       Impact factor: 4.330

5.  Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.

Authors:  K Numazaki; S Chiba; T Yamanaka; T Moroboshi; K Aoki; T Nakao
Journal:  J Clin Pathol       Date:  1985-07       Impact factor: 3.411

6.  Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults.

Authors:  J B Mahony; M A Chernesky; K Bromberg; J Schachter
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

7.  IgA antibodies in HLA-B27 associated acute anterior uveitis and ankylosing spondylitis.

Authors:  S H Sprenkels; J Uksila; R Vainionpää; P Toivanen; T E Feltkamp
Journal:  Clin Rheumatol       Date:  1996-01       Impact factor: 2.980

8.  Diagnosis of Chlamydia trachomatis infection--culture versus serology.

Authors:  E Schoenwald; B L Schmidt; G Steinmetz; J Hosmann; G Pohla-Gubo; A Luger; G Gasser
Journal:  Eur J Epidemiol       Date:  1988-03       Impact factor: 8.082

9.  Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.

Authors:  K Numazaki; S Chiba; T Moroboshi; T Kudoh; T Yamanaka; T Nakao
Journal:  J Clin Pathol       Date:  1985-03       Impact factor: 3.411

Review 10.  Laboratory diagnosis of human chlamydial infections.

Authors:  R C Barnes
Journal:  Clin Microbiol Rev       Date:  1989-04       Impact factor: 26.132
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