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Literature DB >> 7266664

Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.

M Konarska, W Filipowicz, H Domdey, H J Gross.

Abstract

Extracts of wheat germ contain an RNA ligase activity that catalyses the conversation of linear polyribonucleotides into covalently closed circles. The newly formed 3',5'-phosphodiester bridge is resistant to alkali and a number of ribonucleases. This unusual stability is due to the presence of a phosphoryl group esterified to the 2'-position of the 3'-nucleotide joined. This is the first demonstration of an RNA ligase in higher plants and of a natural 2'-phosphomonoester, 3',5'-phosphodiester linkage.

Entities: Chemical Species

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Year: 1981 PMID： 7266664 DOI： 10.1038/293112a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

47 in total

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9. Uridine insertion into preedited mRNA by a mitochondrial extract from Leishmania tarentolae: stereochemical evidence for the enzyme cascade model.

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10. A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.

Authors: S L Spinelli; H S Malik; S A Consaul; E M Phizicky
Journal: Proc Natl Acad Sci U S A Date: 1998-11-24 Impact factor: 11.205

Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.

1. Evidence for a single rolling circle in the replication of potato spindle tuber viroid.

2. Characterization of the intron-encoded U19 RNA, a new mammalian small nucleolar RNA that is not associated with fibrillarin.

3. Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.

4. RNA-mediated ligation of self-cleavage products of a Neurospora mitochondrial plasmid transcript.

5. Organization and transient expression of the gene for human U11 snRNA.

6. Conserved mechanism of tRNA splicing in eukaryotes.

7. The structure of an archaeal homodimeric ligase which has RNA circularization activity.

8. A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.

9. Uridine insertion into preedited mRNA by a mitochondrial extract from Leishmania tarentolae: stereochemical evidence for the enzyme cascade model.

10. A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.