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Literature DB >> 7264323

Serological identification of viral antigens after electrophoretic transfer.

D Karcher, A Lowenthal, H Thormar, M Noppe.

Abstract

The method described permits detection of specific antibodies to antigenic material, the protein components of which can first be separated by electrophoresis and then transferred to a supporting medium where their reactivity with antibody may be demonstrated using peroxidase-labelled anti-Ig. Results are described for measles virus and measles-like virus isolated from subacute sclerosing panencephalitis (SSPE) brain. Dilutions, incubation time and conditions, detection with peroxidase-labelled immunoglobulins and selection of substrate are described and discussed.

Entities: Disease Species

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Year: 1981 PMID： 7264323 DOI： 10.1016/0022-1759(81)90021-1

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

11 in total

1. Characterization of BADS-binding proteins in epithelial plasma membranes.

Authors: S F Pearce; J A Zadunaisky
Journal: J Membr Biol Date: 1991-09 Impact factor: 1.843

2. Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the expression of embryoglycan.

Authors: P Dráber; J Nosek; Z Pokorná
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

3. A serum protein affecting the regulation of the immune system.

Authors: D Karcher; A Lowenthal; P Stoppie; M Noppe
Journal: Neurochem Res Date: 1984-10 Impact factor: 3.996

4. Genetic polymorphism of CON 1 and CON 2 salivary proteins detected by immunologic and concanavalin A reactions on nitrocellulose with linkage of CON 1 and CON 2 genes to the SPC (salivary protein gene complex).

Authors: E A Azen; P L Yu
Journal: Biochem Genet Date: 1984-02 Impact factor: 1.890

9. Bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from Aeromonas hydrophila.

Authors: J M Rose; C W Houston; A Kurosky
Journal: Infect Immun Date: 1989-04 Impact factor: 3.441

10. Occurrence of bovine herpesvirus-1 DNA in nucleosomes and chromatin of bovine herpesvirus-1-infected cells: identification of a virion-associated protein in chromatin of infected cells.

Authors: B S Seal; J D Martinez; M R Hall; S C St Jeor
Journal: Arch Virol Date: 1988 Impact factor: 2.574

Serological identification of viral antigens after electrophoretic transfer.

1. Characterization of BADS-binding proteins in epithelial plasma membranes.

2. Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the expression of embryoglycan.

3. A serum protein affecting the regulation of the immune system.

4. Genetic polymorphism of CON 1 and CON 2 salivary proteins detected by immunologic and concanavalin A reactions on nitrocellulose with linkage of CON 1 and CON 2 genes to the SPC (salivary protein gene complex).

5. Quantification of bands produced by isoelectric focusing using immunoperoxidase.

6. Experimental allergic myositis: ultrastructural, histochemical, immunological and immunohistochemical studies.

7. Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11.

8. Subacute sclerosing panencephalitis antibodies against measles virus polypeptides.

9. Bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from Aeromonas hydrophila.

10. Occurrence of bovine herpesvirus-1 DNA in nucleosomes and chromatin of bovine herpesvirus-1-infected cells: identification of a virion-associated protein in chromatin of infected cells.