Literature DB >> 6386892

Quantification of bands produced by isoelectric focusing using immunoperoxidase.

U H Paluch, G Keir, S Moyle, E J Thompson.   

Abstract

An improved, rapid, and sensitive (to autoradiographic levels) method for the quantification of IgG bands from cerebrospinal fluid is presented. The principle of an internal standard is employed using the discrete bands of a paraprotein to correct for any run to run variability in the enzyme detection system which is linked to the immunoassay of immobilised antigen.

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Year:  1984        PMID： 6386892      PMCID： PMC498961          DOI： 10.1136/jcp.37.10.1172

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  13 in total

1.  Demonstration of electrophoretically restricted virus-specific antibodies in serum and cerebrospinal fluid by imprint electroimmunofixation.

Authors:  H J Nordal; B Vandvik; E Norrby
Journal:  Scand J Immunol       Date:  1978       Impact factor: 3.487

2.  Agarose gel electrophoresis.

Authors:  J O Jeppsson; C B Laurell; B Franzén
Journal:  Clin Chem       Date:  1979-04       Impact factor: 8.327

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  A comparative study of three approaches to the routine quantitative determination of spinal fluid total proteins.

Authors:  B Karlsson; C Alling
Journal:  Clin Chim Acta       Date:  1980-07-17       Impact factor: 3.786

5.  A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining.

Authors:  R W Walker; G Keir; M H Johnson; E J Thompson
Journal:  J Neuroimmunol       Date:  1983-04       Impact factor: 3.478

6.  Agarose isoelectric focusing of unconcentrated CSF and radioimmunofixation for detection of oligoclonal bands in patients with multiple sclerosis and other neurological diseases.

Authors:  V K Kostulas; H Link
Journal:  J Neurol Sci       Date:  1982-04       Impact factor: 3.181

7.  Measurement of body fluid proteins by polyacrylamide gel electrophoresis.

Authors:  M H Johnson; E J Thompson
Journal:  J Clin Pathol       Date:  1982-12       Impact factor: 3.411

8.  Detection of IgE- and IgG-binding proteins after electrophoretic transfer from polyacrylamide gels.

Authors:  R Sutton; C W Wrigley; B A Baldo
Journal:  J Immunol Methods       Date:  1982-07-30       Impact factor: 2.303

9.  Serological identification of viral antigens after electrophoretic transfer.

Authors:  D Karcher; A Lowenthal; H Thormar; M Noppe
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

10.  Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex.

Authors:  R H Yolken; F J Leister; L S Whitcomb; M Santosham
Journal:  J Immunol Methods       Date:  1983-02-11       Impact factor: 2.303
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