Literature DB >> 7251154

Characterization of the temperature sensitive phenotype of the A/Ann Arbor/6/60 cold-adapted virus and its recombinants.

B R Murphy, H F Maassab, F T Wood, R M Chanock.   

Abstract

The A/Ann Arbor/6/60 cold-adapted (ca) donor virus had a 38 degrees C shutoff temperature when tested in MDCK tissue culture. ca recombinant viruses bearing all six "internal genes" of the A/Ann Arbor/6/60 ca donor virus and the surface antigens of wild-type virus can manifest a 1,000-fold difference in plaquing efficiency at 38 degrees C. These observations suggest that the A/Ann Arbor/6/60 ca donor genes that specify the temperature sensitive phenotype of the ca recombinants can undergo genetic modification during the production and passage of the recombinants. The NS gene in the ca recombinant virus could be inherited from either parent without influencing the level of temperature sensitivity of the ca recombinant.

Entities:  

Mesh：

Year:  1981        PMID： 7251154      PMCID： PMC351536          DOI： 10.1128/iai.32.2.960-963.1981

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  18 in total

1.  Adaptation and growth characteristics of influenza virus at 25 degrees c.

Authors:  H F Maassab
Journal:  Nature       Date:  1967-02-11       Impact factor: 49.962

2.  A placebo-controlled dose-response study of the reactogenicity and immunogenicity of a cold-adapted recombinant A/Victoria/3/75 (H3N2) live influenza virus candidate vaccine in healthy volunteers.

Authors:  A Hrabar; I Vodopija; F E André; J R Mitchell; H F Maassab; A V Hennessy; F M Davenport
Journal:  Dev Biol Stand       Date:  1977 Jun 1-3

3.  Laboratory and clinical characteristics of attenuated strains of influenza virus.

Authors:  H F Maassab; T Francis; F M Davenport; A V Hennessy; E Minuse; G Anderson
Journal:  Bull World Health Organ       Date:  1969       Impact factor: 9.408

4.  Biologic and immunologic characteristics of cold-adapted influenza virus.

Authors:  H F Maassab
Journal:  J Immunol       Date:  1969-03       Impact factor: 5.422

5.  Cold-adapted variants of influenza virus A. I. Comparison of the genetic properties of ts mutants and five cold-adapted variants of influenza virus A.

Authors:  S B Spring; H F Maassab; A P Kendal; B R Murphy; R M Chanock
Journal:  Virology       Date:  1977-03       Impact factor: 3.616

6.  Pilot studies on recombinant cold-adapted live type A and B influenza virus vaccines.

Authors:  F M Davenport; A V Hennessy; H F Maassab; E Minuse; L C Clark; G D Abrams; J R Mitchell
Journal:  J Infect Dis       Date:  1977-07       Impact factor: 5.226

7.  Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25 degrees between recent H3N2 and H1N1 epidemic strains and cold-adapted A/An Arbor/6/60.

Authors:  N J Cox; H F Maassab; A P Kendal
Journal:  Virology       Date:  1979-08       Impact factor: 3.616

8.  Cold adapted variants of influenza A. II. Comparison of the genetic and biological properties of ts mutants and recombinants of the cold adapted A/AA/6/60 strain.

Authors:  S B Spring; H F Maassab; A P Kendal; B R Murphy; R M Chanock
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574

9.  Cold-adapted variants of influenza A virus: evaluation in adult seronegative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60 strain.

Authors:  B R Murphy; H P Holley; E J Berquist; M M Levine; S B Spring; H F Maassab; A P Kendal; R M Chanock
Journal:  Infect Immun       Date:  1979-02       Impact factor: 3.441

10.  Relationship of genotype of recombinants of influenza A/Hong Kong/68-ts-1[E]virus used as live virus vaccines to virulence in humans.

Authors:  L J Markoff; F Thierry; B R Murphy; R M Chanock
Journal:  Infect Immun       Date:  1979-10       Impact factor: 3.441
View more
  3 in total

1.  Attenuation of wild-type human influenza A virus by acquisition of the PA polymerase and matrix protein genes of influenza A/Ann Arbor/6/60 cold-adapted donor virus.

Authors:  M H Snyder; M L Clements; D De Borde; H F Maassab; B R Murphy
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

2.  Production and level of genetic stability of an influenza A virus temperature-sensitive mutant containing two genes with ts mutations.

Authors:  B R Murphy; L J Markoff; N T Hosier; J G Massicot; R M Chanock
Journal:  Infect Immun       Date:  1982-07       Impact factor: 3.441

3.  A Mutated PB1 Residue 319 Synergizes with the PB2 N265S Mutation of the Live Attenuated Influenza Vaccine to Convey Temperature Sensitivity.

Authors:  Andrew Cox; Jordana Schmierer; Josephine D'Angelo; Andrew Smith; Dustyn Levenson; John Treanor; Baek Kim; Stephen Dewhurst
Journal:  Viruses       Date:  2020-10-31       Impact factor: 5.048
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.