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Literature DB >> 7242526

Highly purified ceruloplasmin messenger RNA from rat liver. Physico-chemical and functional characteristics.

V S Gaitskhoki, V M L'vov, L V Puchkova, A L Schwartzman, S A Neifakh.

Abstract

Highly purified ceruloplasmin mRNA was isolated from rat liver polyribosomes. The molecular weight of ceruloplasmin mRNA is in a range from 1.05 to 1.25 . 10(6) daltons which is large enough to code for a putative precursor of ceruloplasmin (approximately 700 amino acid acids). Ceruloplasmin mRNA contains 3'-terminal poly(A) the length of which varies from 38 to 165 nucleotides. The 5'-end of ceruloplasmin mRNA is blocked with confronting m7G residue which is a component of cap I (m7G5'ppp5'XmpAp). The addition of ceruloplasmin mRNA to wheat-germ cell free system programmed the synthesis of a product that was largely precipitated by anti-ceruloplasmin immunoglobulins. The translation product was homogeneous in polyacrylamide gel-sodium dodecylsulfate electrophoresis. Cell-free translation of ceruloplasmin mRNA was sensitive to inhibition by cap analogue.

Entities: Chemical Gene Species

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Year: 1981 PMID： 7242526 DOI： 10.1007/bf02357087

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

29 in total

1. The messenger RNA's for the liver enzymes tyrosine aminotransferase and tryptophan oxygenase contain 40-50 % non-coding sequences.

Authors: E Hofer; C E Sekeris
Journal: Biochem Biophys Res Commun Date: 1977-07-11 Impact factor: 3.575

5. Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells.

Authors: I Schechter
Journal: Biochemistry Date: 1974-04-23 Impact factor: 3.162

10. Modified nucleosides and 5'-end groups in purified mouse immunoglobulin light chain mRNA and rabbit globin mRNA detected by borohydride labelling.

Authors: S Cory; C Genin; J M Adams
Journal: Biochim Biophys Acta Date: 1976-12-01

1 in total

1. Identification of ceruloplasmin messenger RNA sequences in heterogeneous nuclear RNA from rat liver.

Authors: V S Gaitskhoki; V M L'vov; A L Schwartzman; N A Skobeleva; L J Frolova; S A Neifakh
Journal: Mol Biol Rep Date: 1981-11-30 Impact factor: 2.316

1 in total

Highly purified ceruloplasmin messenger RNA from rat liver. Physico-chemical and functional characteristics.

1. The messenger RNA's for the liver enzymes tyrosine aminotransferase and tryptophan oxygenase contain 40-50 % non-coding sequences.

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

3. On the defect of synthesis ceruloplasmin in the liver polyribosomes in Wilson's disease.

4. Molecular pathology of ceruloplasmin.

5. Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells.

6. Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. Functions of the 5,-terminal m7G cap in eukaryotic mRNA.

9. Isolation of rat liver albumin messenger RNA.

10. Modified nucleosides and 5'-end groups in purified mouse immunoglobulin light chain mRNA and rabbit globin mRNA detected by borohydride labelling.

1. Identification of ceruloplasmin messenger RNA sequences in heterogeneous nuclear RNA from rat liver.