Literature DB >> 7242149

An improved method for the bulk isolation of viable perikarya from postnatal cerebellum.

G R Dutton, D N Currie, K Tear.   

Abstract

A modified method for the isolation of large numbers of viable perikarya from postnatally developing rat cerebellum is described in comparison with an earlier published version. Advantages of the modified preparation include greater cell yields which emphasize the preservation of the larger sized perikarya (inhibitory interneurons and Purkinje cells). In addition, the use of cytotoxic EDTA is eliminated, and by combining several steps and using a simpler buffer system, the preparation time is reduced by half to less than 1 h.

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Year:  1981        PMID： 7242149     DOI： 10.1016/0165-0270(81)90029-7

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  17 in total

1.  Alignment of astrocytes increases neuronal growth in three-dimensional collagen gels and is maintained following plastic compression to form a spinal cord repair conduit.

Authors:  Emma East; Daniela Blum de Oliveira; Jon P Golding; James B Phillips
Journal:  Tissue Eng Part A       Date:  2010-10       Impact factor: 3.845

2.  A novel protein, amyloid precursor-like protein 2, is present in human brain, cerebrospinal fluid and conditioned media.

Authors:  M T Webster; N Groome; P T Francis; B R Pearce; F E Sherriff; G Thinakaran; K M Felsenstein; W Wasco; R E Tanzi; D M Bowen
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

3.  Predominance of the acylation route in the metabolic processing of exogenous sphingosine in neural and extraneural cells in culture.

Authors:  L Riboni; R Bassi; A Prinetti; P Viani; G Tettamanti
Journal:  Biochem J       Date:  1999-02-15       Impact factor: 3.857

4.  Ca2+ signals mediated by P2X-type purinoceptors in cultured cerebellar Purkinje cells.

Authors:  J Mateo; M García-Lecea; M T Miras-Portugal; E Castro
Journal:  J Neurosci       Date:  1998-03-01       Impact factor: 6.167

5.  K+-evoked taurine efflux from cerebellar astrocytes: on the roles of Ca2+ and Na+.

Authors:  R A Philibert; K L Rogers; G R Dutton
Journal:  Neurochem Res       Date:  1989-01       Impact factor: 3.996

6.  GABA neurones in retinas of different species and their postnatal development in situ and in culture in the rabbit retina.

Authors:  N N Osborne; S Patel; D W Beaton; V Neuhoff
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

7.  K(+)-stimulated amino acid release from cultured cerebellar neurons: comparison of static and dynamic stimulation paradigms.

Authors:  K L Rogers; R A Philibert; G R Dutton
Journal:  Neurochem Res       Date:  1991-08       Impact factor: 3.996

8.  Cellular uptake disguises action of L-glutamate on N-methyl-D-aspartate receptors. With an appendix: diffusion of transported amino acids into brain slices.

Authors:  J Garthwaite
Journal:  Br J Pharmacol       Date:  1985-05       Impact factor: 8.739

9.  Cultured cerebellar granule cells, but not astrocytes, produce an ester of ganglioside GD1b, presumably GD1b monolactone, from exogenous GD1b.

Authors:  R Bassi; L Riboni; G Tettamanti
Journal:  Biochem J       Date:  1994-09-15       Impact factor: 3.857

10.  A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis.

Authors:  Emma East; Jonathan P Golding; James B Phillips
Journal:  J Tissue Eng Regen Med       Date:  2009-12       Impact factor: 3.963
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